Gabpb1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:96483 O'Leary DA, et al., Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1. Gene. 2005 Jan 3;344:79-92
Assay type:	RT-PCR
MGI Accession ID:	MGI:5817578
Gene symbol:	Gabpb1
Gene name:	GA repeat binding protein, beta 1

Probe:	Beta1f, B1-1r
Assay notes:	The RT-PCR products were blotted and hybridized with an internal oligonucleotide probe MGI:5817575.

Results

Image: 6A bottom

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	953 bp (a)	841 bp (b)	762 bp (c)	233 bp (d)	Amount	Genetic Background	Mutant Allele(s)	Sex
Stomach +	P adult	TS28: stomach	Present	Absent	Absent	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Stomach -	Control
Large Int. +	P adult	TS28: large intestine	Present	Absent	Absent	Present	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Large Int. -	Control
Small Int. +	P adult	TS28: small intestine	Present	Absent	Present	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Small Int. -	Control
Fat +	P adult	TS28: adipose tissue	Present	Absent	Absent	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Fat -	Control
Sk. mus +	P adult	TS28: skeletal muscle	Present	Absent	Present	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Sk. mus -	Control
Tongue +	P adult	TS28: tongue	Present	Absent	Present	Present	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Tongue -	Control
Eyes +	P adult	TS28: eye	Present	Absent	Present	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Eyes -	Control
Testis +	P adult	TS28: testis	Present	Absent	Absent	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Male
Testis -	Control
Epidid. +	P adult	TS28: epididymis	Present	Absent	Absent	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Male
Epidid. -	Control
Ovary/Uterus +	P adult	TS28: ovary TS28: uterus	Present	Absent	Absent	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Female
Ovary/Uterus -	Control

Notes:
(a) Full length transcript. Gabp-beta1-1 isoform. Expression level differed between tissues.
(b) Splice form - exon 7-9.
(c) Splice form - exon 3-5.
(d) Splice form - exon 3-9.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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