Probe:	CRABP-II cDNA
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent		1A

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	1B	Expressed throughout the neuroepithelium except in the floor plate region at the midline of the neural groove.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural fold	Present		1C	Stronger expression.
TS12: neural ectoderm	Present	Regionally restricted	1C	Staining was stronger in the neuroepithelium of the presumptive hindbrain region. More diffuse labeling extended caudally lateral to the primitive streak region.
TS12: primitive streak	Present	Diffuse	1C

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Widespread	1D	Expressed throughout the length of the neuroepithelium.
TS12: rhombomere	Present	Regionally restricted	1D	Stronger expression extended caudally from the rhombomere B/rhombomere C boundary and gradually diminished over the length of the embryo with no distinguishable posterior boundary.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 1	Moderate		1E
TS13: rhombomere 2	Moderate		1E
TS13: rhombomere 3	Moderate		1E
TS13: rhombomere 4	Present		1E	Relatively high level, approximately equivalent to that in rhombomere 6.
TS13: 1st branchial arch	Weak		1E	Expressed in a band extending from rhombomere 1/2 to the first branchial arch.
TS13: 2nd branchial arch	Weak		1E	Expressed in a band extending from rhombomere 4 to the second branchial arch.
TS13: posterior pro-rhombomere	Present	Regionally restricted	1E	Relatively high level expression in rhombomere 6.
TS13: future midbrain	Weak		1E
TS13: future prosencephalon	Weak		1E
TS13: rhombomere 5	Weak		1E

Structure	Level	Pattern	Image	Note
TS14: future diencephalon	Moderate		1F
TS14: future telencephalon	Moderate		1F
TS14: future midbrain	Weak		1F
TS14: fronto-nasal process	Weak	Regionally restricted	1F	Expressed in the mesenchyme.
TS14: 1st branchial arch	Present		1F
TS14: 2nd branchial arch	Present		1F
TS14: rhombomere 1	Present		1F
TS14: rhombomere 2	Present		1F
TS14: rhombomere 3	Present		1F
TS14: rhombomere 4	Present		1F
TS14: rhombomere 5	Present		1F
TS14: rhombomere 6	Present		1F

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Widespread	3A	Expressed throughout the length of the neuroepithelium.
TS12: rhombomere	Present	Regionally restricted	3A	Stronger expression extended caudally from the rhombomere B/rhombomere C boundary and gradually diminished over the length of the embryo with no distinguishable posterior boundary.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 1	Moderate		3B
TS13: rhombomere 2	Moderate		3B
TS13: rhombomere 3	Moderate		3B
TS13: rhombomere 4	Present		3B	Relatively high level, approximately equivalent to that in rhombomere 6.
TS13: 1st branchial arch	Weak		3B	Expressed in a band extending from rhombomere 1/2 to the first branchial arch.
TS13: 2nd branchial arch	Weak		3B	Expressed in a band extending from rhombomere 4 to the second branchial arch.
TS13: posterior pro-rhombomere	Present	Regionally restricted	3B	Relatively high level expression in rhombomere 6.
TS13: future midbrain	Weak		3B
TS13: future prosencephalon	Weak		3B
TS13: rhombomere 5	Weak		3B

Structure	Level	Pattern	Image	Note
TS13: rhombomere 1	Moderate		3C
TS13: rhombomere 2	Moderate		3C
TS13: rhombomere 3	Moderate		3C
TS13: rhombomere 4	Present		3C	Relatively high level, approximately equivalent to that in rhombomere 6.
TS13: 1st branchial arch	Weak		3C	Expressed in a band extending from rhombomere 1/2 to the first branchial arch.
TS13: 2nd branchial arch	Weak		3C	Expressed in a band extending from rhombomere 4 to the second branchial arch.
TS13: posterior pro-rhombomere	Present	Regionally restricted	3C	Relatively high level expression in rhombomere 6.
TS13: future midbrain	Weak		3C
TS13: future prosencephalon	Weak		3C
TS13: rhombomere 5	Weak		3C

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		4B
TS15: telencephalon	Present		4B
TS15: olfactory placode	Absent		4B
TS15: diencephalon	Present		4B	Expression decreased in the diencephalon to mesencephalic levels.
TS15: fronto-nasal process mesenchyme	Moderate		4B	Moderate to low.
TS15: limb bud	Moderate		4B	Moderate to low.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		4E
TS15: telencephalon	Present		4E
TS15: olfactory placode	Absent		4E
TS15: diencephalon	Present		4E	Expression decreased in the diencephalon to mesencephalic levels.
TS15: fronto-nasal process mesenchyme	Moderate		4E	Moderate to low.
TS15: limb bud	Moderate		4E	Moderate to low.