Gene Expression Data
Assay Details
Assay
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Reference:
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J:15507
Dolle P, et al., Disruption of the Hoxd-13 gene induces localized heterochrony leading to mice with neotenic limbs. Cell. 1993 Nov 5;75(3):431-41
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5816729
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Gene symbol:
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Hoxd13
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Gene name:
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homeobox D13
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Probe:
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probe A
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 11.5
(more )
Specimen
5A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: footplate |
Present |
Regionally restricted |
5A
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Authors report strong and sharply defined expression in the extremities of both fore- and hindlimbs.
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TS19: handplate |
Present |
Regionally restricted |
5A
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Authors report strong and sharply defined expression in the extremities of both fore- and hindlimbs.
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Specimen
5B:
embryonic day 12.5
(more )
Specimen
5B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: foot |
Present |
Regionally restricted |
5B
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Authors report strong and sharply defined expression in the extremities of both fore- and hindlimbs.
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TS20: hand |
Present |
Regionally restricted |
5B
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Authors report strong and sharply defined expression in the extremities of both fore- and hindlimbs.
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Specimen
5C:
embryonic day 12.5
(more )
Specimen
5C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5D:
embryonic day 12.5
(more )
Specimen
5D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: foot mesenchyme |
Present |
Regionally restricted |
5D
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Expression in most of posterior-distal mesenchymal cells.
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TS20: hindlimb digit 1 |
Absent |
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5D
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Expression domain not extended to digit 1 anlage.
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS22: forelimb digit 1 |
Present |
Regionally restricted |
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In older handplates, expression detected up to area of prospective digit 1.
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TS22: hand mesenchyme |
Present |
Regionally restricted |
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Expression domain restricted to perichondrium.
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