Gene Expression Data
Assay Details
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Assay
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Reference:
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J:99651
Hartwig S, et al., Glypican-3 modulates inhibitory Bmp2-Smad signaling to control renal development in vivo. Mech Dev. 2005 Jul-Aug;122(7-8):928-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5816256
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Gene symbol:
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Smad1
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Gene name:
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SMAD family member 1
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Antibody:
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anti-phospho-SMAD1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
6A Wt:
embryonic day 13.5
(more  )
Specimen
6A Wt:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: metanephros |
Strong |
Regionally restricted |
6A Wt
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Strong nuclear expression in both ureteric bud and mesenchymal compartments.
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Specimen
6A Bmp2+/-:
embryonic day 13.5; Bmp2tm1Brd/Bmp2 +
(more )
Specimen
6A Bmp2+/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Bmp2tm1Brd/Bmp2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: metanephros |
Strong |
Regionally restricted |
6A Bmp2+/-
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Strong nuclear expression in both ureteric bud and mesenchymal compartments.
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Specimen
6A Gpc3+/-:
embryonic day 13.5; Gpc3tm1Fil/Gpc3 +
(more )
Specimen
6A Gpc3+/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Gpc3tm1Fil/Gpc3+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: metanephros |
Strong |
Regionally restricted |
6A Gpc3+/-
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Strong nuclear expression in both ureteric bud and mesenchymal compartments.
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Specimen
6A Gpc3+/-;Bmp2+/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Bmp2tm1Brd/Bmp2+
Gpc3tm1Fil/Gpc3+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: metanephros |
Weak |
Regionally restricted |
6A Gpc3+/-;Bmp2+/-
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Expression reduced in both ureteric bud and mesenchyme compared to wild type and single mutants.
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Analysis Tools
