Gene Expression Data
Assay Details
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Assay
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Reference:
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J:99651
Hartwig S, et al., Glypican-3 modulates inhibitory Bmp2-Smad signaling to control renal development in vivo. Mech Dev. 2005 Jul-Aug;122(7-8):928-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5816254
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Gene symbol:
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Gpc3
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Gene name:
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glypican 3
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Antibody:
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glypican-3 antibody (1G12)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Authors describe two glypican-3 monoclonal antibodies; MGI:5816244 may have been used.
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Results
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Specimen
4 Wt:
embryonic day 13.5
(more  )
Specimen
4 Wt:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ureteric tip |
Strong |
Widespread |
4_Wt
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Strong expression throughout the ureteric bud (membrane and cytoplasmic staining).
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| TS21: ureteric trunk |
Strong |
Widespread |
4_Wt
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Strong expression throughout the ureteric bud (membrane and cytoplasmic staining).
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Specimen
4 Bmp2+/-:
embryonic day 13.5; Bmp2tm1Brd/Bmp2 +
(more )
Specimen
4 Bmp2+/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Bmp2tm1Brd/Bmp2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ureteric tip |
Strong |
Widespread |
4_Bmp2+/-
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Strong expression throughout the ureteric bud (membrane and cytoplasmic staining).
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| TS21: ureteric trunk |
Strong |
Widespread |
4_Bmp2+/-
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Strong expression throughout the ureteric bud (membrane and cytoplasmic staining).
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Specimen
4 Gpc3+/- (upper):
embryonic day 13.5; Gpc3tm1Fil/Gpc3 +
(more )
Specimen
4 Gpc3+/- (upper):
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Gpc3tm1Fil/Gpc3+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ureteric trunk |
Present |
Regionally restricted |
4_Gpc3+/- (upper)
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Authors report mosaic expression in ureteric buds, with expression in some cells of ureteric bud tips and trunks but undetectable in others.
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Specimen
4 Gpc3+/- (lower):
embryonic day 13.5; Gpc3tm1Fil/Gpc3 +
(more )
Specimen
4 Gpc3+/- (lower):
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Gpc3tm1Fil/Gpc3+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ureteric trunk |
Present |
Regionally restricted |
4_Gpc3+/- (lower)
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Authors report mosaic expression in ureteric buds, with expression in some cells of ureteric bud tips and trunks but undetectable in others.
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| TS21: ureteric tip |
Ambiguous |
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4_Gpc3+/- (lower)
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Authors report mosaic expression in ureteric buds, with expression in some cells of ureteric bud tips and trunks but undetectable in others.
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Analysis Tools
