Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:210059
Szenasi T, et al., Hmgb1 can facilitate activation of the matrilin-1 gene promoter by Sox9 and L-Sox5/Sox6 in early steps of chondrogenesis. Biochim Biophys Acta. 2013 Oct;1829(10):1075-91
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5816097
|
|
Gene symbol:
|
Matn1
|
|
Gene name:
|
matrilin 1, cartilage matrix protein
|
|
Antibody:
|
anti-Matn1
|
|
Detection system:
|
Secondary antibody coupled to Cy3
|
|
|
Results
|
Specimen
5C:
embryonic day 14.5
(more  )
Note: Double labeled: green - Matn1; red - Hmgb1 (assay MGI:5816095).
Specimen
5C:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Acetone
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Matn1; red - Hmgb1 (assay MGI:5816095).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: metatarsus cartilage condensation |
Strong |
Regionally restricted |
5C Matn1
,
5C merge
|
Expression is in differentiated cartilaginous elements and is weak in early chondroblasts of developing metatarsals.
|
| TS22: foot mesenchyme |
Present |
Regionally restricted |
5C merge
,
5C Matn1
|
Expression is in differentiated cartilaginous elements and is weak in early chondroblasts of developing metatarsals and is absent in other foot mesenchyme.
|
Specimen
5E:
embryonic day 14.5
(more )
Note: Double labeled: green - Matn1; red - Hmgb1 (assay MGI:5816095).
Specimen
5E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Acetone
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Matn1; red - Hmgb1 (assay MGI:5816095).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: metatarsus cartilage condensation |
Strong |
Regionally restricted |
5E merge
,
5E Matn1
|
Expression is in differentiated cartilaginous elements and is weak in early chondroblasts of developing metatarsals.
|
| TS22: foot mesenchyme |
Present |
Regionally restricted |
5E Matn1
,
5E merge
|
Expression is in differentiated cartilaginous elements and is weak in early chondroblasts of developing metatarsals and is absent in other foot mesenchyme.
|
|
Analysis Tools