Gene Expression Data
Assay Details
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Assay
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Reference:
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J:117267
Sarkisian MR, et al., MEKK4 signaling regulates filamin expression and neuronal migration. Neuron. 2006 Dec 7;52(5):789-801
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5812486
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Gene symbol:
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Nes
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Gene name:
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nestin
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Antibody:
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clone RC2
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Detection system:
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Secondary antibody
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Results
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Specimen
4A'/A":
embryonic day 17.5
(more  )
Note: Double labeled: red - Nes; green - Laminin.
Specimen
4A'/A":
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Nes; green - Laminin.
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Specimen
4B'/B":
embryonic day 17.5; Map3k4tm1Flv/ Map3k4tm1Flv
(more )
Note: Highly degenerated forebrain mutant sample. Double labeled: red - Nes; green - Laminin.
Specimen
4B'/B":
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Map3k4tm1Flv/Map3k4tm1Flv
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Highly degenerated forebrain mutant sample. Double labeled: red - Nes; green - Laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cerebral cortex |
Present |
Regionally restricted |
4B'
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4B"
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Expressed in radial glial cells. The morphology and organization was markedly disrupted at sites where laminin was disrupted.
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| TS25: cerebral cortex ventricular zone |
Present |
Regionally restricted |
4B'
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4B"
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Expressed in radial glial cells. The morphology and organization was markedly disrupted at sites where laminin was disrupted.
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Specimen
4D/D':
embryonic day 17.5; Map3k4tm1Flv/ Map3k4tm1Flv
(more )
Note: Highly degenerated forebrain mutant sample. Double labeled: red - Nes; green - Laminin.
Specimen
4D/D':
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Map3k4tm1Flv/Map3k4tm1Flv
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Highly degenerated forebrain mutant sample. Double labeled: red - Nes; green - Laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cerebral cortex |
Present |
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4D
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4D'
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Expressed in radial glial cells. In place of an ectopia, disrupted pia label and extension of positive fibers into the ectopia were shown.
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Specimen
S5 left:
embryonic day 18.5; Map3k4tm1Flv/ Map3k4tm1Flv
(more )
Note: highly degenerated forebrain mutant sample
Specimen
S5 left:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Map3k4tm1Flv/Map3k4tm1Flv
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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highly degenerated forebrain mutant sample
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
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S5_left
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Breaks in the lining of the ventricular surface were associated with disrupted radial glia. Positive fibers navigated around disrupted areas to establish an organized pattern of radial glial fibers in the cortical plate.
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| TS26: cortical plate |
Present |
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S5_left
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Specimen
S5 middle:
embryonic day 18.5; Map3k4tm1Flv/ Map3k4tm1Flv
(more )
Note: highly degenerated forebrain mutant sample
Specimen
S5 middle:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Map3k4tm1Flv/Map3k4tm1Flv
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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highly degenerated forebrain mutant sample
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
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S5_middle
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Breaks in the lining of the ventricular surface were associated with disrupted radial glia. Positive fibers navigated around disrupted areas to establish an organized pattern of radial glial fibers in the cortical plate.
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| TS26: cortical plate |
Present |
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S5_middle
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Specimen
S5 right:
embryonic day 18.5; Map3k4tm1Flv/ Map3k4tm1Flv
(more )
Note: highly degenerated forebrain mutant sample
Specimen
S5 right:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Map3k4tm1Flv/Map3k4tm1Flv
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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highly degenerated forebrain mutant sample
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
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S5_right
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Breaks in the lining of the ventricular surface were associated with disrupted radial glia. Positive fibers navigated around disrupted areas to establish an organized pattern of radial glial fibers in the cortical plate.
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| TS26: cortical plate |
Present |
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S5_right
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Specimen
not shown:
embryonic day 18.5
(more )
Note: Double labeled: red - Nes; green - Laminin.
Specimen
not shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Nes; green - Laminin.
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Specimen
not shown:
embryonic day 18.5; Map3k4tm1Flv/ Map3k4tm1Flv
(more )
Note: Highly degenerated forebrain mutant sample. Double labeled: red - Nes; green - Laminin.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Map3k4tm1Flv/Map3k4tm1Flv
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Highly degenerated forebrain mutant sample. Double labeled: red - Nes; green - Laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
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Expressed in radial glial cells. The morphology and organization was markedly disrupted at sites where laminin was disrupted.
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| TS26: forebrain ventricular layer |
Present |
Regionally restricted |
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Expressed in radial glial cells. The morphology and organization was markedly disrupted at sites where laminin was disrupted.
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