Probe:	Opa3 probe1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS17: head	Present	Ubiquitous	1A

Structure	Level	Pattern	Image	Note
TS19: head	Present	Ubiquitous	1B

Structure	Level	Pattern	Image	Note
TS22: salivary gland	Present	Regionally restricted	1Ci	Expression was detected in submandibular salivary glands.

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	1Cii	Expression was detected in developing mandibular bone surrounding Meckel's cartilage.
TS22: Meckel's cartilage	Absent		1Cii

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	1Ciii	Expression was detected in developing mandibular bone surrounding Meckel's cartilage.
TS22: Meckel's cartilage	Absent		1Ciii

Structure	Level	Pattern	Image	Note
TS22: lower jaw incisor	Present		1Civ

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar	Present		1Cv
TS22: upper jaw molar	Present		1Cv

Structure	Level	Pattern	Image	Note
TS22: upper jaw incisor	Present		1Cvi

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	1Cvii	Expression was detected in the sensory layer of the retina.

Structure	Level	Pattern	Image	Note
TS26: hair follicle	Present		1Di

Structure	Level	Pattern	Image	Note
TS26: brain	Present		1Dii
TS26: cranium	Ambiguous		1Dii

Structure	Level	Pattern	Image	Note
TS26: upper jaw molar	Present	Regionally restricted	1Diii	Expression was detected in upper molar tooth germ. Strong expression was noted in odontoblasts, while weak expression was seen in ameloblasts.

Structure	Level	Pattern	Image	Note
TS26: incisor	Present	Regionally restricted	1Div	In the incisors the immature odontoblasts showed strong expression whereas Opa3 was weakly expressed in mature odontoblasts at E18.5. In contrast, strong expression was observed in ameloblasts in both anterior and posterior regions of incisors at E18.5.

Structure	Level	Pattern	Image	Note
TS26: incisor	Present	Regionally restricted	1Dv	In the incisors the immature odontoblasts showed strong expression whereas Opa3 was weakly expressed in mature odontoblasts at E18.5. In contrast, strong expression was observed in ameloblasts in both anterior and posterior regions of incisors at E18.5.

Structure	Level	Pattern	Image	Note
TS26: incisor	Present	Regionally restricted	1Dvi	In the incisors the immature odontoblasts showed strong expression whereas Opa3 was weakly expressed in mature odontoblasts at E18.5. In contrast, strong expression was observed in ameloblasts in both anterior and posterior regions of incisors at E18.5.

Structure	Level	Pattern	Image	Note
TS26: incisor	Present	Regionally restricted	1Dvii	In the incisors the immature odontoblasts showed strong expression whereas Opa3 was weakly expressed in mature odontoblasts at E18.5. In contrast, strong expression was observed in ameloblasts in both anterior and posterior regions of incisors at E18.5.