Cdh6 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:45678 Henderson DJ, et al., Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch). Mech Dev. 1997 Dec;69(1-2):39-51
Assay type:	RNA in situ
MGI Accession ID:	MGI:5811766
Gene symbol:	Cdh6
Gene name:	cadherin 6

Probe:	cadherin-6 probe3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

5A wt ©

Specimen 5A wt: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	5A wt	Expression in in ventral forebrain region.
TS15: 1st branchial arch	Present		5A wt
TS15: 2nd branchial arch	Present		5A wt
TS15: branchial arch	Present	Regionally restricted	5A wt	Expression in cells migrating through branchial arches 4 and 6, and at more caudal levels.

5A Sp2H ©

Specimen 5A Sp2H: embryonic day 9.5; Pax3^Sp-2H/Pax3^Sp-2H (more

)

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		5A Sp2H	Expression similar to wild type in branchial arches 1 and 2, and in ventral forebrain region. No expression in branchial arches 4 and 6, or at more caudal levels.
TS15: 2nd branchial arch	Present		5A Sp2H	Expression similar to wild type in branchial arches 1 and 2, and in ventral forebrain region. No expression in branchial arches 4 and 6, or at more caudal levels.
TS15: future forebrain	Present	Regionally restricted	5A Sp2H	Expression in in ventral forebrain region.

5B ©

Specimen 5B: embryonic day 9.5 (more

)
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	5B	Expression in neural crest cells migrating through paraxial mesoderm.
TS15: paraxial mesenchyme	Absent		5B	No expression in surrounding paraxial mesoderm.

5C ©

Specimen 5C: embryonic day 9.5; Pax3^Sp-2H/Pax3^Sp-2H (more

)
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Absent		5C	No expression in migrating cells.
TS15: neural crest	Present	Regionally restricted	5C	Expression instead in a clumps of cells lateral to the dorsal region of the neural tube.

5D ©

Specimen 5D: embryonic day 9.5 (more

)
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	5D	Abundant expressing cells surrounding the aortic sac just superior to the outflow tract.

5E ©

Specimen 5E: embryonic day 9.5; Pax3^Sp-2H/Pax3^Sp-2H (more

)
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Absent		5E	No expression in cells surrounding the aortic sac just superior to the outflow tract.

Specimen E10.5 wt not shown: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial arch	Present

Specimen E10.5 Sp2H not shown: embryonic day 10.5; Pax3^Sp-2H/Pax3^Sp-2H (more

)

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial arch	Present			Expression in branchial arch 3 similar in wild type and mutant embryos.
TS17: 4th branchial arch	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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