Probe:	versican V1 probe A
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Probe A was used which detects V1 splice variants. Authors also report using a second probe, probe B (MGI:5811760) corresponding to V2 variant. Both probes detect the full length unspliced V0 transcript and yielded identical patterns of expression.

Structure	Level	Pattern	Image	Note
TS14: somite	Weak	Regionally restricted		Low levels of expression in somitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Regionally restricted		Expression in dorsal mesoderm immediately adjacent to neural tube.
TS15: sclerotome	Present			Expression maintained in sclerotome.
TS15: dermomyotome	Absent			Authors report expression is down-regulated in dermomyotome.

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Regionally restricted	2A wt	Expression in mesoderm of occipital/cervical region.
TS15: somite	Present	Regionally restricted	2A wt	Most intense expression in rostral somites; lower levels of expression in more caudal somites.
TS15: neural tube	Present	Regionally restricted	2A wt	In neural tube, expression exhibits cranio-caudal variation.
TS15: caudal neuropore	Present		2A wt
TS15: 1st branchial arch	Present		2A wt
TS15: 2nd branchial arch	Present		2A wt
TS15: 3rd branchial arch	Present		2A wt
TS15: hindgut	Present		2A wt

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Strong	Regionally restricted	2A Sp2H	Overexpression, particularly in the occipital/cervical region, in mesoderm lateral to hindbrain.
TS15: somite	Strong	Regionally restricted	2A Sp2H	Most pronounced difference in rostral somites.
TS15: 1st branchial arch	Present		2A Sp2H	Marginally more expression in mutant compared to wild type.
TS15: 2nd branchial arch	Present		2A Sp2H	Expression appear closely similar in mutant and wild type embryos.
TS15: 3rd branchial arch	Present		2A Sp2H	Expression appear closely similar in mutant and wild type embryos.
TS15: branchial arch	Present	Regionally restricted	2A Sp2H	Expression in lower branchial arches 4 amd 6 (through which NCC migrate to the heart).

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Regionally restricted	2B	At upper cervical region, expression in mesoderm lateral to neural tube and dorsal to developing dermomyotome.
TS15: sclerotome	Present		2B
TS15: neural tube	Present	Regionally restricted	2B	No expression in cervical neural tube.
TS15: migrating neural crest	Absent		2B	No expression in bilateral streams of neural crest cells migrating away from the neural tube, migrating through positively expressing somites.
TS15: foregut	Absent		2B

Structure	Level	Pattern	Image	Note
TS15: paraxial mesenchyme	Strong	Widespread	2C	Markedly increased expression throughout paraxial mesoderm compared with wildtype., with no evidence of nonexpressing migrating cells.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Widespread	2D	In low spinal level, expression throughout presomitic mesoderm.
TS15: tail neural tube	Present	Widespread	2D	In caudal regions of neural tube, marked expression throughout the neuroepithelium.
TS15: hindgut	Present		2D

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present		2E	No marked differences in expression between wild type and mutant embryos at this level of the body.
TS15: tail neural tube	Present		2E	No marked differences in expression between wild type and mutant embryos at this level of the body.
TS15: tail unsegmented mesenchyme	Present		2E	No marked differences in expression between wild type and mutant embryos at this level of the body.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Regionally restricted	3A wt	Expression in occipital/cervical and cervical mesoderm.
TS17: somite	Present	Regionally restricted	3A wt	Expression in caudal part of each somites.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Strong	Regionally restricted	3A Sp2H	Over-expression in dorsal mesoderm of occipital cervical region and in the most rostral somites.
TS17: somite	Present	Regionally restricted	3A Sp2H	Expression in caudal part of each somite.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Strong	Regionally restricted	3B	Highest levels of expression in mesoderm dorsolateral to dorsal root ganglia and ventromedial to dermomyotome.
TS17: dermomyotome	Absent		3B	In the upper cervical region, no expression in dermomyotome and dorsal root ganglia.
TS17: dorsal root ganglion	Absent		3B	In the upper cervical region, no expression in dermomyotome and dorsal root ganglia.
TS17: sclerotome	Present	Regionally restricted	3B	Expression in sclerotome adjacent to and surrounding the notochord.

Structure	Level	Pattern	Image	Note
TS17: paraxial mesenchyme	Strong	Regionally restricted	3C	Strong expression in mesoderm immediately lateral to the dorsal neural tube, where neural crest cells first exit (the dorsal wedge. Ectopic expression, with infilling in regions where ventral dermomyotome and dorsal root ganglia would normally be situated.

Structure	Level	Pattern	Image	Note
TS17: paraxial mesenchyme	Strong	Regionally restricted	3D	Highest levels of expression in mesoderm dorsolateral to dorsal root ganglia and ventromedial to dermomyotome.
TS17: dermomyotome	Absent		3D
TS17: dorsal root ganglion	Absent		3D
TS17: sclerotome	Present	Regionally restricted	3D	Expression in sclerotome adjacent to and surrounding the notochord.
TS17: trunk mesenchyme	Strong	Regionally restricted	3D	Strong expression in domain immediately lateral to dermomyotome where dorsal surface of limb is continuous with the flank. Expression localised to small trangular area where the limb bud joins the flank.

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Present	Regionally restricted	3E	Expression in the sclerotome closely similar in wild type and mutant embryo.
TS17: forelimb bud	Present	Regionally restricted	3E	Ectopic expression in the dorsal part of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	3F	Expression confined to caudal part of each somite.

Structure	Level	Pattern	Image	Note
TS19: embryo mesenchyme	Present	Regionally restricted	4A wt	Highest expression in dorsal mesoderm adjacent to neural tube and lateral to dorsal root ganglia.
TS19: dorsal root ganglion	Absent		4A wt
TS19: somite	Present	Regionally restricted	4A wt	Expression in caudal part of each somite.
TS19: limb bud	Present		4A wt

Structure	Level	Pattern	Image	Note
TS19: dermomyotome	Strong	Regionally restricted	4A Sp2H	In embryo with a large spina bifida, intense expression at the level of the hindlimb bud in area occupied by dermomyotome.

Structure	Level	Pattern	Image	Note
TS19: paraxial mesenchyme	Present	Regionally restricted	4B	Expression in dorsal paraxial mesoderm.
TS19: dermomyotome	Absent		4B	No expression in dermomyotome and dorsal root ganglia.
TS19: dorsal root ganglion	Absent		4B	No expression in dermomyotome and dorsal root ganglia.
TS19: sclerotome	Ambiguous		4B	Expression downregulated in sclerotome and around notochord.
TS19: forelimb bud	Present	Regionally restricted	4B	Clearly demarcated domain of expression at dorsal margin of forelimb bud.

Structure	Level	Pattern	Image	Note
TS19: paraxial mesenchyme	Strong	Regionally restricted	4C	Enlarged domain of expression extending from the dorsal-most paraxial mesoderm to the dorsal limb bud.
TS19: dermomyotome	Present	Regionally restricted	4C	Ectopic expression in dermomyotome and dorsal root ganglia.
TS19: dorsal root ganglion	Present	Regionally restricted	4C	Ectopic expression in dermomyotome and dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS17: paraxial mesenchyme	Strong	Regionally restricted	6B	Strong expression in mesoderm dorso-medial to dermomyotome.
TS17: dermomyotome	Absent		6B	Expression is excluded from dermomyotome.
TS17: sclerotome	Present		6B	Expression in sclerotome and perinotochordal mesoderm.
TS17: limb mesenchyme	Strong	Regionally restricted	6B	Intense expression in dorsal part of limb bud where it extends from flank.

Structure	Level	Pattern	Image	Note
TS17: paraxial mesenchyme	Present	Regionally restricted	6D	Authors describe infilling of expression in regions normally expressing Pax3, such as the dermomytome, mesoderm lateral to dorsal part of neural tube, and site of dorsal root ganglia.
TS17: neural tube	Absent		6D	No upregulation of expression in dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS19: paraxial mesenchyme	Strong	Regionally restricted	7B	Strong expression in mesoderm dorso-medial to dermomyotome.
TS19: dermomyotome	Absent		7B
TS19: dorsal root ganglion	Absent		7B
TS19: sclerotome	Present		7B	Expression in sclerotome and perinotochordal mesoderm.
TS19: limb mesenchyme	Strong	Regionally restricted	7B	Intense expression in dorsal part of limb bud where it extends from flank.

Structure	Level	Pattern	Image	Note
TS19: paraxial mesenchyme	Present	Regionally restricted	7D	Authors report infilling of expression where Pax3 would normally be expressed.
TS19: dermomyotome	Present	Regionally restricted	7D	Ectopic expression in dermomytome and dorsal-most part of dorsal root ganglia.
TS19: dorsal root ganglion	Present	Regionally restricted	7D	Ectopic expression in dermomytome and dorsal-most part of dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS20: somite	Present

Structure	Level	Pattern	Image	Note
TS20: somite	Present	Regionally restricted		Abnormal expression in the somites, with separation of expression domains in adjacent somites appearing less distinct than in wild type embryos.