Ghrl RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:83705 Kawamura K, et al., Ghrelin inhibits the development of mouse preimplantation embryos in vitro. Endocrinology. 2003 Jun;144(6):2623-33
Assay type:	RT-PCR
MGI Accession ID:	MGI:5811294
Gene symbol:	Ghrl
Gene name:	ghrelin

Probe:	Ghrelin-pC, Ghrelin-pD
Assay notes:	Hemi-nested PCR was performed with first amplification using primers Ghrelin-pA, Grhrelin-pB (MGI:5811288, expected size of product 394 bp) and 2nd amplification using primers Ghrelin-pC, Ghrelin-pD (MGI:5811289, expected size of product 350 bp).

Results

Image: 1_ghrelin

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	350 bp (a)	239 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
marker	Mol. Wt. Marker Lane
positive control	P adult	TS28: placenta	Present	Present	Not Specified; poly-A+ RNA	IVCS		Female
negative control	Control
oocyte	P adult	TS28: secondary oocyte	Absent	Absent	Not Specified; poly-A+ RNA	Not Specified		Female
1-cell	E0.5 (c)	TS1: 1-cell stage embryo	Absent	Absent	Not Specified; poly-A+ RNA	IVCS		Not Specified
2-cell	E1.0 (c)	TS2: 2-cell stage embryo	Absent	Absent	Not Specified; poly-A+ RNA	IVCS		Not Specified
4-cell	In vitro data: no data stored
8-cell	In vitro data: no data stored
morula	In vitro data: no data stored
blastocyst	In vitro data: no data stored
hatched blastocyst	In vitro data: no data stored

Notes:
(a) Corresponds to expected proghrelin amplification product.
(b) Represent a novel proghrelin fragment with a C-terminal truncation.
(c) Age assigned by curator based on morphological criteria supplied by authors.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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