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Gene Expression Data
Assay Details
Assay
Reference: J:83705 Kawamura K, et al., Ghrelin inhibits the development of mouse preimplantation embryos in vitro. Endocrinology. 2003 Jun;144(6):2623-33
Assay type: RT-PCR
MGI Accession ID: MGI:5811294
Gene symbol: Ghrl
Gene name: ghrelin
Probe: Ghrelin-pC, Ghrelin-pD
Assay notes: Hemi-nested PCR was performed with first amplification using primers Ghrelin-pA, Grhrelin-pB (MGI:5811288, expected size of product 394 bp) and 2nd amplification using primers Ghrelin-pC, Ghrelin-pD (MGI:5811289, expected size of product 350 bp).
Results Image: 1_ghrelin
 Sample Information Bands Other Sample Information
Lane AgeStructure 350 bp (a) 239 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex
marker Mol. Wt. Marker Lane
positive control P adult TS28: placenta Present Present Not Specified; poly-A+ RNA IVCS Female
negative control Control
oocyte P adult TS28: secondary oocyte Absent Absent Not Specified; poly-A+ RNA Not Specified Female
1-cell E0.5 (c) TS1: 1-cell stage embryo Absent Absent Not Specified; poly-A+ RNA IVCS Not Specified
2-cell E1.0 (c) TS2: 2-cell stage embryo Absent Absent Not Specified; poly-A+ RNA IVCS Not Specified
4-cell In vitro data: no data stored
8-cell In vitro data: no data stored
morula In vitro data: no data stored
blastocyst In vitro data: no data stored
hatched blastocyst In vitro data: no data stored
Notes:
(a) Corresponds to expected proghrelin amplification product.
(b) Represent a novel proghrelin fragment with a C-terminal truncation.
(c) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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