Msx2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:195918 Vieux-Rochas M, et al., BMP-mediated functional cooperation between Dlx5;Dlx6 and Msx1;Msx2 during mammalian limb development. PLoS One. 2013;8(1):e51700
Assay type:	RNA in situ
MGI Accession ID:	MGI:5810611
Gene symbol:	Msx2
Gene name:	msh homeobox 2

Probe:	Msx2 probe30
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

2Q ©

Specimen 2Q: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: forelimb bud apical ectodermal ridge	Present		2Q
TS18: forelimb bud mesenchyme	Present		2Q	Expression in mesoderm underlying the AER.

2R ©

Specimen 2R: embryonic day 11.0; Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi (more

)
Note: This embryo was a single homozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud apical ectodermal ridge	Trace		2R	Expression drastically reduced or absent in the AER.
TS18: hindlimb bud mesenchyme	Weak	Regionally restricted	2R	Expression strongly reduced or absent in a central wedge of mesoderm underlying AER, but not in a proximal mesodermal territory.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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