Ccna2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:63193 Winston N, et al., Early development of mouse embryos null mutant for the cyclin A2 gene occurs in the absence of maternally derived cyclin A2 gene products. Dev Biol. 2000 Jul 1;223(1):139-53
Assay type:	RT-PCR
MGI Accession ID:	MGI:5810272
Gene symbol:	Ccna2
Gene name:	cyclin A2

Probe:	RTA2U, RTA2L
Assay notes:	These data are described in the text. The PCR was Southern blotted and hybridized with a probe generated from reverse transcribed intestinal RNA using the same primers.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	352 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
+/+	P	TS28: secondary oocyte	Present (a)	Not Specified	involves: 129P2/OlaHsd * BALB/c		Female	Metaphase II oocytes.
+/-	P	TS28: secondary oocyte	Present (a)	Not Specified	involves: 129P2/OlaHsd * BALB/c	Ccna2^tm1Sot/Ccna2⁺	Female	Metaphase II oocytes.
-/-	P	TS28: secondary oocyte	Present (a)	Not Specified	involves: 129P2/OlaHsd * BALB/c	Ccna2^tm1Sot/Ccna2^tm1Sot	Female	Metaphase II oocytes.
+/+	E1.0 (b)	TS2: 2-cell stage conceptus	Present (c)	Not Specified	involves: 129P2/OlaHsd * BALB/c		Not Specified
+/-	E1.0 (b)	TS2: 2-cell stage conceptus	Present (c)	Not Specified	involves: 129P2/OlaHsd * BALB/c	Ccna2^tm1Sot/Ccna2⁺	Not Specified
-/-	E1.0 (b)	TS2: 2-cell stage conceptus	Present (c)	Not Specified	involves: 129P2/OlaHsd * BALB/c	Ccna2^tm1Sot/Ccna2^tm1Sot	Not Specified
+/+	E3.5	TS4: blastocyst-stage conceptus	Present (d)	Not Specified	involves: 129P2/OlaHsd * BALB/c		Not Specified
+/-	E3.5	TS4: blastocyst-stage conceptus	Present (d)	Not Specified	involves: 129P2/OlaHsd * BALB/c	Ccna2^tm1Sot/Ccna2⁺	Not Specified
-/-	E3.5	TS4: blastocyst-stage conceptus	Absent (d)	Not Specified	involves: 129P2/OlaHsd * BALB/c	Ccna2^tm1Sot/Ccna2^tm1Sot	Not Specified

Notes:
(a) RNA in the oocyte is maternally deposited.
(b) Age assigned by curator based on morphological criteria supplied by authors.
(c) Zygotic transcription has yet to begin.
(d) At this time point maternally deposited RNA has been depleted and zygotic transcription has begun.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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