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Gene Expression Data
Assay Details
Assay
Reference: J:63193 Winston N, et al., Early development of mouse embryos null mutant for the cyclin A2 gene occurs in the absence of maternally derived cyclin A2 gene products. Dev Biol. 2000 Jul 1;223(1):139-53
Assay type: RT-PCR
MGI Accession ID: MGI:5810272
Gene symbol: Ccna2
Gene name: cyclin A2
Probe: RTA2U, RTA2L
Assay notes: These data are described in the text. The PCR was Southern blotted and hybridized with a probe generated from reverse transcribed intestinal RNA using the same primers.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 352 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
+/+ P TS28: secondary oocyte Present (a) Not Specified involves: 129P2/OlaHsd * BALB/c Female Metaphase II oocytes.
+/- P TS28: secondary oocyte Present (a) Not Specified involves: 129P2/OlaHsd * BALB/c Ccna2tm1Sot/Ccna2+Female Metaphase II oocytes.
-/- P TS28: secondary oocyte Present (a) Not Specified involves: 129P2/OlaHsd * BALB/c Ccna2tm1Sot/Ccna2tm1SotFemale Metaphase II oocytes.
+/+ E1.0 (b) TS2: 2-cell stage conceptus Present (c) Not Specified involves: 129P2/OlaHsd * BALB/c Not Specified
+/- E1.0 (b) TS2: 2-cell stage conceptus Present (c) Not Specified involves: 129P2/OlaHsd * BALB/c Ccna2tm1Sot/Ccna2+Not Specified
-/- E1.0 (b) TS2: 2-cell stage conceptus Present (c) Not Specified involves: 129P2/OlaHsd * BALB/c Ccna2tm1Sot/Ccna2tm1SotNot Specified
+/+ E3.5 TS4: blastocyst-stage conceptus Present (d) Not Specified involves: 129P2/OlaHsd * BALB/c Not Specified
+/- E3.5 TS4: blastocyst-stage conceptus Present (d) Not Specified involves: 129P2/OlaHsd * BALB/c Ccna2tm1Sot/Ccna2+Not Specified
-/- E3.5 TS4: blastocyst-stage conceptus Absent (d) Not Specified involves: 129P2/OlaHsd * BALB/c Ccna2tm1Sot/Ccna2tm1SotNot Specified
Notes:
(a) RNA in the oocyte is maternally deposited.
(b) Age assigned by curator based on morphological criteria supplied by authors.
(c) Zygotic transcription has yet to begin.
(d) At this time point maternally deposited RNA has been depleted and zygotic transcription has begun.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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