Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:63193
Winston N, et al., Early development of mouse embryos null mutant for the cyclin A2 gene occurs in the absence of maternally derived cyclin A2 gene products. Dev Biol. 2000 Jul 1;223(1):139-53
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5810134
|
|
Gene symbol:
|
Ccna2
|
|
Gene name:
|
cyclin A2
|
|
Antibody:
|
anti-cyclin A2
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
|
Results
|
Specimen
4C:
postnatal adult
(more  )
Note: Metaphase II oocyte. Confocal image.
Specimen
4C:
(close  )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Female
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Metaphase II oocyte. Confocal image.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Present |
|
4C
|
Expression is at two distinct intensely stained groups of points located at an equal distance either side of the metaphase chromatin and at possible microtubule-organizing centers.
|
Specimen
5A:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/c
|
|
Mutant Allele(s):
|
Ccna2tm1Sot/Ccna2tm1Sot
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Confocal image
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Absent |
|
5A
|
Twelve percent of 8-cell stage embryos derived from heterozygotic crosses showed no staining (and thus were presumed to be homozygotes).
|
Specimen
5B/C:
embryonic day 2.5; Ccna2tm1Sot/Ccna2 +
(more )
Note: Confocal image
Specimen
5B/C:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/c
|
|
Mutant Allele(s):
|
Ccna2tm1Sot/Ccna2+
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Confocal image
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
|
5C
,
5B
|
This embryo was derived from a heterozygotic cross and thus could have been wild type.
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/c
|
|
Mutant Allele(s):
|
Ccna2tm1Sot/Ccna2tm1Sot
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Confocal image
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Absent |
|
5D
|
Twelve percent of 8-cell stage embryos derived from heterozygotic crosses showed no staining (and thus were presumed to be homozygotes).
|
Specimen
5E/F:
embryonic day 2.5; Ccna2tm1Sot/Ccna2 +
(more )
Note: Confocal image
Specimen
5E/F:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/c
|
|
Mutant Allele(s):
|
Ccna2tm1Sot/Ccna2+
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Confocal image
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
|
5E
,
5F
|
This embryo was derived from a heterozygotic cross and thus could have been wild type.
|
Specimen
not shown - wt:
embryonic day 2.5
(more )
Note: Confocal image
Specimen
not shown - wt:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/c
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Confocal image
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
|
|
This embryo was the result of a heterozygotic cross.
|
Specimen
not shown - wt:
embryonic day 3.0
(more )
Note: Confocal image
Specimen
not shown - wt:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/c
|
|
Age:
|
embryonic day 3.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Confocal image
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
|
|
These embryos were derived from heterozygotic crosses; no individual embryo was genotyped.
|
Specimen
now shown - +/-:
embryonic day 3.0; Ccna2tm1Sot/Ccna2 +
(more )
Note: Confocal image
Specimen
now shown - +/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/c
|
|
Mutant Allele(s):
|
Ccna2tm1Sot/Ccna2+
|
|
Age:
|
embryonic day 3.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Confocal image
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
|
|
These embryos were derived from heterozygotic crosses; no individual embryo was genotyped.
|
Specimen
not shown - -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * BALB/c
|
|
Mutant Allele(s):
|
Ccna2tm1Sot/Ccna2tm1Sot
|
|
Age:
|
embryonic day 3.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Confocal image
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Absent |
|
|
Sixteen percent of morula-stage embryos derived from heterozygotic crosses showed no staining (and thus were presumed to be homozygotes).
|
|
Analysis Tools