Prdm16 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122807 Warner DR, et al., PRDM16/MEL1: a novel Smad binding protein expressed in murine embryonic orofacial tissue. Biochim Biophys Acta. 2007 Jun;1773(6):814-20
Assay type:	Northern blot
MGI Accession ID:	MGI:5807409
Gene symbol:	Prdm16
Gene name:	PR domain containing 16

Probe:	PRDM16/MEL1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Autoradiography

Results

Image: 4A PRDM16/MEL1

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	10 kb (a)	7 kb (b)	5 kb (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E12.5	E12.5	TS20: oral region	Strong	Strong	Strong	10 µg; total RNA	ICR		Not Specified
E13.5	E13.5	TS21: oral region	Present	Present	Present	10 µg; total RNA	ICR		Not Specified
E14.5	E14.5	TS22: oral region	Present	Present	Present	10 µg; total RNA	ICR		Not Specified

Notes:
(a) Major 10 kb transcript detected in each sample. No significant difference in level of expression across this developmental window.
(b) Minor trancripts also present with no apparent difference in levels of expression.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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