Probe:	Hippi probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Ubiquitous	3A	Ubiquitous expression through the epiblast.
TS10: node	Strong		3A	High levels of expression in the node region.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Ubiquitous	3B	Ubiquitous expression through the epiblast.
TS10: node	Strong		3B	High levels of expression in the node region.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Strong		3C	Strong expression in the ectoderm.
TS10: node	Strong		3C	High levels of expression in the node region.
TS10: embryo mesoderm	Present		3C
TS10: embryo endoderm	Absent		3C

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Ubiquitous	3D	Ubiquitous expression through the epiblast.
TS11: node	Strong		3D	High levels of expression in the node region.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Widespread	3E
TS13: future prosencephalon	Strong		3E	Strong expression detected mainly in the forebrain, anterior midline and somites.
TS13: somite	Strong		3E	Strong expression detected mainly in the forebrain, anterior midline and somites.
TS13: heart	Absent		3E

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Widespread	3F
TS13: future prosencephalon	Strong		3F	Strong expression detected mainly in the forebrain, anterior midline and somites.
TS13: future spinal cord	Strong		3F	Strong expression detected mainly in the forebrain, anterior midline and somites.
TS13: somite	Strong		3F	Strong expression detected mainly in the forebrain, anterior midline and somites.
TS13: heart	Absent		3F

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Strong		3G	Expression pattern is maintained at E9.5. Highest expression in the forebrain.
TS15: central nervous system	Present	Widespread	3G	Expression extends to all regions of the neural tube.
TS15: branchial arch	Strong		3G	High expression in branchial arches and limb buds.
TS15: limb bud	Strong		3G	High expression in branchial arches and limb buds.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Strong		3H
TS15: central nervous system	Present	Widespread	3H	Expression throughout the neural tube. Strongest expression on the luminal neural tube.
TS15: embryo mesoderm	Present	Widespread	3H
TS15: foregut	Present	Regionally restricted	3H	Expression in the ventral foregut.
TS15: surface ectoderm	Absent		3H

Structure	Level	Pattern	Image	Note
TS17: forebrain	Strong		3I	Expression pattern is maintained at E10.5. Highest expression in the forebrain.
TS17: central nervous system	Present	Widespread	3I	Expression extends to all regions of the neural tube.
TS17: branchial arch	Strong		3I	High expression in branchial arches and limb buds.
TS17: limb bud	Strong		3I	High expression in branchial arches and limb buds.