Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1(CAG-GCaMP6f)Khakh
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Baljit Khakh
MGI ID:	MGI:5806654
Synonyms:	Lck-GCaMP6fflox, R26-Lck-GCaMP6fflox
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1(CAG-GCaMP6f)Khakh page

Germline Transmission:	Earliest citation of germline transmission: J:249928
Parent Cell Line:	JM8.N4 (ES Cell)
Strain of Origin:	C57BL/6N

Allele Type:    Targeted (Conditional ready, Reporter) Mutation:    Insertion   Mutation details: The targeting vector is designed with (from 5' to 3') with a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), an FRT site, a loxP-flanked STOP cassette, the GCaMP6f coding sequence with N-terminal Lck tag (described below), a WPRE, a BGH polyA signal, and an attB/attP-flanked PGK-frt-Neo-polyA cassette. Lck-GCaMP6f is a GCaMP6 fast variant calcium indicator with an N-terminal Lck-tag that targets GCaMP6f to the plasma membrane. In the absence of calcium binding, dim EGFP fluorescence is expected as there is a pore from the outside of its barrel into the chromophore. Upon calcium binding, this pore becomes occupied and fluorescence is increased. (J:249928)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	944 strains or lines available

Original:	J:249928 Srinivasan R, et al., New Transgenic Mouse Lines for Selectively Targeting Astrocytes and Studying Calcium Signals in Astrocyte Processes In Situ and In Vivo. Neuron. 2016 Dec 21;92(6):1181-1195
All:	8 reference(s)