Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Slc32a1tm1.1(flpo)Hze
Name:	solute carrier family 32 (GABA vesicular transporter), member 1; targeted mutation 1.1, Hongkui Zeng
MGI ID:	MGI:5806634
Synonyms:	Slc32a1-2A-FlpO-D
Gene:	Slc32a1  Location: Chr2:158452687-158457668 bp, + strand  Genetic Position: Chr2, 78.72 cM, cytoband H1-3
Alliance:	Slc32a1tm1.1(flpo)Hze page

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Slc32a1tm1.1(flpo)Hze expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Slc32a1 (MGI:1194488)   Mutation details: The targeting vector contains, from 5' to 3', a partial Slc32a1 sequence spanning exon 1 (with an FRT3 site in intron 1) through part of exon 2 up to (but not including) the endogenous stop codon, a T2A sequence, a flpO recombinase gene (a codon-optimized FLPe gene), a BHG polyA sequence, an AttB site, a PGK-neomycin cassette, an FRT5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL). (J:101977)

Activity:	Tissue activity of this recombinase allele
Driver:	Slc32a1 (mouse) Summary of all recombinase alleles driven by Slc32a1. Alliance mouse Slc32a1 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Slc32a1 Mutation:	54 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	15 reference(s)