Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Gnb4tm1.1(cre)Hze
Name:	guanine nucleotide binding protein (G protein), beta 4; targeted mutation 1.1, Hongkui Zeng
MGI ID:	MGI:5806633
Synonyms:	Gnb4-IRES2-Cre-D
Gene:	Gnb4  Location: Chr3:32634481-32670734 bp, - strand  Genetic Position: Chr3, 15.78 cM, cytoband B
Alliance:	Gnb4tm1.1(cre)Hze page

Germline Transmission:	Earliest citation of germline transmission: J:236597
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Gnb4tm1.1(cre)Hze expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Gnb4 (MGI:104581)   Mutation details: The targeting vector contained, from 5' to 3', a partial Gnb4 sequence spanning intron 6 through intron 9, an frt3 site within Gnb4 intron 9, a partial Gnb4 exon 10 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the the AttB/AttP-flanked PGK-neo sequence and replace it with the recombined AttB/AttP site (AttL). (J:236597)

Activity:	Tissue activity of this recombinase allele
Driver:	Gnb4 (mouse) Summary of all recombinase alleles driven by Gnb4. Alliance mouse Gnb4 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gnb4 Mutation:	30 strains or lines available

Original:	J:236597 Wang Q, et al., Organization of the connections between claustrum and cortex in the mouse. J Comp Neurol. 2017 Apr 15;525(6):1317-1346
All:	2 reference(s)