Nkx6-1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:104952 Pedersen JK, et al., Endodermal expression of Nkx6 genes depends differentially on Pdx1. Dev Biol. 2005 Dec 15;288(2):487-501
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5806620
Gene symbol:	Nkx6-1
Gene name:	NK6 homeobox 1

Antibody:	anti-Nkx6-1
Detection system:	Secondary antibody
Assay notes:	Two antibodies were described but the one used in this assay was not detailed. Nkx6.1 antibody MGI:5806603 may have been used in this assay.

Results

7E ©

Specimen 7E: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS16: pancreas primordium dorsal bud	Present	Single cells	7E	Few expressing cells in dorsal pancreatic epithelium.
TS16: pancreas primordium ventral bud	Absent		7E

7F ©

Specimen 7F: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS16: stomach	Present	Regionally restricted	7F	Expression at level of anterior stomach. Expression in stomach mesenchyme.

7Q ©

Specimen 7Q: embryonic day 10.5 (more

)
Note: Double labeled: red - Nkx6-1; green - Pdx1 (assay MGI:5806621).

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium dorsal bud	Present	Regionally restricted	7Q	Expression in dorsal pancreatic epithelium.
TS17: duodenum	Absent		7Q

8B ©

Specimen 8B: embryonic day 10.5 (more

)
Note: Double labeled: red - Nkx6-1; green - Gcg (assay MGI:5806631).

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium dorsal bud	Present	Regionally restricted	8B	Expression in most of the undifferentiated epithelial cells of both the dorsal and ventral pancreatic buds.

Specimen not shown: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium ventral bud	Present	Regionally restricted		Expression in most of the undifferentiated epithelial cells of both the dorsal and ventral pancreatic buds.

8E ©

Specimen 8E: embryonic day 10.5; Pdx1^tm1Cvw/Pdx1^tm1Cvw (more

)
Note: Double labeled: red - Nkx6-1; green - Gcg (assay MGI:5806631).

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium dorsal bud	Present	Regionally restricted	8E	In the dorsal epithelium, expression in a few cells. No expression in the undifferentiated epithelial cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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