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Gene Expression Data
Assay Details
Assay
Reference: J:193956 Cheung CC, et al., Genetic labeling of steroidogenic factor-1 (SF-1) neurons in mice reveals ventromedial nucleus of the hypothalamus (VMH) circuitry beginning at neurogenesis and development of a separate non-SF-1 neuronal cluster in the ventrolateral VMH. J Comp Neurol. 2013 Apr 15;521(6):1268-88
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5806598
Gene symbol: Nr5a1
Gene name: nuclear receptor subfamily 5, group A, member 1
Reporter: GFP
Antibody: anti-GFP (Aves)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1D/E: postnatal day 0; Nr5a1tm2.1Hain/Nr5a1tm2.1Hain (more )
Note: Double labeled: green - Nr5a1 reporter; red - Nr5a1 protein (assay MGI:5806599).
Structure Level Pattern Image Note
TS27: ventromedial hypothalamic nucleus Strong 1E , 1D Expressed in the soma, colocalized with the staining of the protein, and in neurites.
Specimen 7I/K/L: postnatal day 0; Nr5a1tm2.1Hain/Nr5a1tm2.1Hain (more )
Note: Double labeled: green - Nr5a1 reporter; red - Nr5a1 protein (assay MGI:5806599).
Structure Level Pattern Image Note
TS27: ventromedial hypothalamic nucleus Present Regionally restricted 7L , 7K , 7I Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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