Nr5a1 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:193956 Cheung CC, et al., Genetic labeling of steroidogenic factor-1 (SF-1) neurons in mice reveals ventromedial nucleus of the hypothalamus (VMH) circuitry beginning at neurogenesis and development of a separate non-SF-1 neuronal cluster in the ventrolateral VMH. J Comp Neurol. 2013 Apr 15;521(6):1268-88
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5806598
Gene symbol:	Nr5a1
Gene name:	nuclear receptor subfamily 5, group A, member 1

Reporter:	GFP
Antibody:	anti-GFP (Aves)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1D ©

1E ©

Specimen 1D/E: postnatal day 0; Nr5a1^tm2.1Hain/Nr5a1^tm2.1Hain (more

)
Note: Double labeled: green - Nr5a1 reporter; red - Nr5a1 protein (assay MGI:5806599).

Structure	Level	Pattern	Image	Note
TS27: ventromedial hypothalamic nucleus	Strong		1E , 1D	Expressed in the soma, colocalized with the staining of the protein, and in neurites.

7I ©

7K ©

7L ©

Specimen 7I/K/L: postnatal day 0; Nr5a1^tm2.1Hain/Nr5a1^tm2.1Hain (more

)
Note: Double labeled: green - Nr5a1 reporter; red - Nr5a1 protein (assay MGI:5806599).

Structure	Level	Pattern	Image	Note
TS27: ventromedial hypothalamic nucleus	Present	Regionally restricted	7L , 7K , 7I	Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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