Gene Expression Data
Assay Details
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Assay
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Reference:
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J:193956
Cheung CC, et al., Genetic labeling of steroidogenic factor-1 (SF-1) neurons in mice reveals ventromedial nucleus of the hypothalamus (VMH) circuitry beginning at neurogenesis and development of a separate non-SF-1 neuronal cluster in the ventrolateral VMH. J Comp Neurol. 2013 Apr 15;521(6):1268-88
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5806597
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Gene symbol:
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Nr5a1
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Gene name:
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nuclear receptor subfamily 5, group A, member 1
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Reporter:
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GFP
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Antibody:
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anti-GFP (Aves)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein
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Results
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Specimen
1B:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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postnatal
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 10.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 10.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forebrain |
Present |
Regionally restricted |
2C
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Expressed in fibers extending into the medial forebrain bundle and traveling toward the epithalamus.
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Specimen
3A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: median eminence |
Present |
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3C
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| TS20: forebrain |
Present |
Regionally restricted |
3C
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Expressed in fibers extending into the medial forebrain bundle, and the caudal, lateral postoptic area. Positive fibers travel medial to the optic tract and lateral to the medial forebrain bundle, following a path similar to the presumptive ventral supraoptic commissure.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Present |
Regionally restricted |
3E
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Expressed in the presumptive ventromedial nucleus of the hypothalamus and fibers, with positive neurons occupying the lateral half of the hypothalamus and extending to the edge of the developing brain.
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Specimen
4A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: median eminence |
Present |
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4A
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| TS22: diencephalon |
Present |
Regionally restricted |
4A
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Expressed in fibers in the median eminence and extending into the ventral supraoptic commissure.
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Specimen
4C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
4C
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Expressed in neurons in the ventromedial nucleus of the hypothalamus.
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| TS22: diencephalon |
Present |
Regionally restricted |
4C
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Expressed in fibers extending dorsally along the ventral supraoptic commissure. A second, scattered group of fibers traverse through the medial forebrain bundle, ascending laterally and dorsolaterally in a fan-like pattern.
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Specimen
4E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
4E
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Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.
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| TS22: diencephalon |
Present |
Regionally restricted |
4E
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Expressed in fibers traveling in the medial forebrain bundle angle toward and join the ventral supraoptic commissure at the level of the external medullary lamina. Most of these fibers travel along the lateral wall of the developing brain and branch toward the epithalamus.
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Specimen
4G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
4G
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Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.
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Specimen
6A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
6E
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Expressed in neurons in the ventromedial nucleus (with an absence of positive cells in the most lateral aspect) and ventral supraoptic commissure of the hypothalamus.
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| TS25: forebrain |
Present |
Regionally restricted |
6E
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Expressed in fibers emerging from the dorsallateral aspect of the nucleus and travel parallel to the ventral supraoptic commissure at the lateral aspect of the medial lemniscus.
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Specimen
6G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
6G
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Expressed in neurons in the ventromedial nucleus (with an absence of positive cells in the most lateral aspect) and ventral supraoptic commissure of the hypothalamus.
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| TS25: forebrain |
Present |
Regionally restricted |
6G
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Expressed in fibers emerging from the dorsallateral aspect of the nucleus and travel parallel to the ventral supraoptic commissure at the lateral aspect of the medial lemniscus.
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Specimen
6I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
|
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
6I
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Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.
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| TS25: forebrain |
Present |
Regionally restricted |
6I
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Expressed in fibers originates from the posterior aspect of the ventromedial nucleus and follows the periventricular system extending along the midline of the brain to traverse through the dorsomedial nucleus and posterior hypothalamus to reach the periaqueductal gray region.
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Specimen
6K:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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postnatal day 0
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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postnatal day 0
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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postnatal day 0
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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postnatal day 0
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: ventromedial hypothalamic nucleus |
Present |
Regionally restricted |
7G
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Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm2.1Hain/Nr5a1tm2.1Hain
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Age:
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postnatal
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools
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