Antibody:

anti-Pecam

Structure	Level	Pattern	Image	Note
TS12: cardinal vein	Present	Regionally restricted	2c	Expression is in the vascular endothelium.
TS12: dorsal aorta	Present	Regionally restricted	2c	Expression is in the vascular endothelium.

Structure	Level	Pattern	Image	Note
TS13: cardinal vein	Present	Regionally restricted	2d	Expression is in the vascular endothelium.
TS13: dorsal aorta	Present	Regionally restricted	2d	Expression is in the vascular endothelium.

Structure	Level	Pattern	Image	Note
TS14: cardinal vein	Present	Regionally restricted	2e	Expression is in the vascular endothelium.
TS14: dorsal aorta	Present	Regionally restricted	2e	Expression is in the vascular endothelium.

Structure	Level	Pattern	Image	Note
TS12: cardinal vein	Present	Regionally restricted	2f	Expression is present in the malformed arteries and veins of this mutant.
TS12: dorsal aorta	Present	Regionally restricted	2f	Expression is present in the malformed arteries and veins of this mutant.

Structure	Level	Pattern	Image	Note
TS13: cardinal vein	Present	Regionally restricted	2g	Expression is present in the malformed arteries and veins of this mutant.
TS13: dorsal aorta	Present	Regionally restricted	2g	Expression is present in the malformed arteries and veins of this mutant.

Structure	Level	Pattern	Image	Note
TS14: cardinal vein	Present	Regionally restricted	2h	Expression is present in the malformed arteries and veins of this mutant.
TS14: dorsal aorta	Present	Regionally restricted	2h	Expression is present in the malformed arteries and veins of this mutant.

Structure	Level	Pattern	Image	Note
TS13: vascular plexus	Present	Regionally restricted	3a	Expression is in the endothelium of the perineural plexus.
TS13: artery	Present	Regionally restricted	3a	Expression is in the endothelium of the intersomitic vessels.
TS13: branchial arch artery	Present	Regionally restricted	3a	Expression is in the endothelium.
TS13: dorsal aorta	Present	Regionally restricted	3a	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS15: vascular plexus	Present	Regionally restricted	3b	Expression is in the endothelium of the perineural plexus.
TS15: branchial arch artery	Present	Regionally restricted	3b	Expression is in the endothelium.
TS15: dorsal aorta	Present	Regionally restricted	3b	Expression is in the endothelium.
TS15: intersomitic artery	Present	Regionally restricted	3b	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS13: vascular plexus	Present	Regionally restricted	3c	Expression is in the endothelium of the perineural plexus.
TS13: artery	Present	Regionally restricted	3c	Expression is in the endothelium of the intersomitic vessels.
TS13: branchial arch artery	Present	Regionally restricted	3c	Expression is in the endothelium.
TS13: dorsal aorta	Present	Regionally restricted	3c	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS15: branchial arch artery	Present	Regionally restricted	3d	Expression is in the endothelium illustrating defects in endothelial remodelling in this mutant.
TS15: dorsal aorta	Present	Regionally restricted	3d	Expression is in the endothelium illustrating defects in endothelial remodelling in this mutant.
TS15: intersomitic artery	Present	Regionally restricted	3d	Expression is in the endothelium illustrating defects in endothelial remodelling in this mutant.
TS15: vascular plexus	Present	Regionally restricted	3d	Expression is in the endothelium illustrating defects in endothelial remodelling in this mutant.
TS15: common cardinal vein	Present	Regionally restricted	3d	Endothelial staining illustrates a dilated vessel in this mutant.

Structure	Level	Pattern	Image	Note
TS16: dorsal aorta	Present	Regionally restricted	5a , 5b	Expression is in the hematopoietic clusters budding from the hemogenic endothelium of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS13: common cardinal vein	Present	Regionally restricted	5d , 5e	Expression is in the endothelium which shows anomalous cluster formation in this mutant.