Reporter:	alkaline phosphatase
	Direct Detection

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present		2A left	Expression first detected at the advanced primitive streak stage as a ring-shaped stripe in the extraembryonic mesoderm of the yolk sac.

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present		2A center left	Expression first detected at the advanced primitive streak stage as a ring-shaped stripe in the extraembryonic mesoderm of the yolk sac.

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present		2A right	Expression first detected at the advanced primitive streak stage as a ring-shaped stripe in the extraembryonic mesoderm of the yolk sac.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Diffuse	2B center right	During neural plate formation, expression domains expanded throughout the yolk sac, becoming more diffuse, and extending into the embryo proper.
TS11: yolk sac	Present	Diffuse	2B center right	During neural plate formation, expression domains expanded throughout the yolk sac, becoming more diffuse, and extending into the embryo proper.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Diffuse	2B right	During neural plate formation, expression domains expanded throughout the yolk sac, becoming more diffuse, and extending into the embryo proper.
TS11: yolk sac	Present	Diffuse	2B right	During neural plate formation, expression domains expanded throughout the yolk sac, becoming more diffuse, and extending into the embryo proper.

Structure	Level	Pattern	Image	Note
TS13: allantois	Strong	Widespread	2C left	Strong expression throughout the embryo proper and the allantois.
TS13: embryo	Strong	Widespread	2C left	Strong expression throughout the embryo proper and the allantois.
TS13: yolk sac	Weak	Regionally restricted	2C left	Lesser staining in the yolk sac membranes.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	2D top	Expression persisted but decreased overall.
TS15: branchial arch	Present	Regionally restricted	2D top	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS15: head mesenchyme	Present	Regionally restricted	2D top	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS15: limb bud	Present	Regionally restricted	2D top	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS15: paraxial mesenchyme	Present	Regionally restricted	2D top	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS15: yolk sac mesenchyme	Present	Regionally restricted	2D top	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	2E	Expression persisted but decreased overall.
TS17: branchial arch	Present	Regionally restricted	2E	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS17: head mesenchyme	Present	Regionally restricted	2E	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS17: limb bud	Present	Regionally restricted	2E	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS17: paraxial mesenchyme	Present	Regionally restricted	2E	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	2F	Expression persisted but decreased overall.
TS19: branchial arch	Present	Regionally restricted	2F	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS19: head mesenchyme	Present	Regionally restricted	2F	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS19: limb bud	Present	Regionally restricted	2F	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS19: paraxial mesenchyme	Present	Regionally restricted	2F	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.
TS19: yolk sac mesenchyme	Present	Regionally restricted	2F	Expression more clustered in the vascular plexus of the cephalic mesenchyme, between the somites, in the branchial arches and limb buds, and in the yolk sac mesenchyme.

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present	Regionally restricted	3aA	Expression restricted to extraembryonic mesoderm (exm) of the visceral yolk sac.
TS10: embryo mesoderm	Present	Regionally restricted	3aA	Expression in anterior-most intraembryonic mesoderm (em) of future heart anlage, located between the amnion and the foregut indentation.
TS10: embryo ectoderm	Absent		3aA	No expression in other regions of embryo proper.
TS10: embryo endoderm	Absent		3aA	No expression in other regions of embryo proper.
TS10: allantois	Absent		3aA	No expression in extraembryonic endoderm of visceral yolk sac, amnion , allantois or ectoplacental cone.
TS10: amniotic fold	Absent		3aA	No expression in extraembryonic endoderm of visceral yolk sac, amnion , allantois or ectoplacental cone.
TS10: ectoplacental cone	Absent		3aA	No expression in extraembryonic endoderm of visceral yolk sac, amnion , allantois or ectoplacental cone.
TS10: yolk sac endoderm	Absent		3aA	No expression in extraembryonic endoderm of visceral yolk sac, amnion , allantois or ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present		3aB	Expression restricted to extraembryonic mesoderm (exm) of the visceral yolk sac.
TS10: embryo mesoderm	Present	Regionally restricted	3aB	Expression in anterior-most intraembryonic mesoderm (em) of future heart anlage, located between the amnion and the foregut indentation.

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present	Regionally restricted	3aC	Expression restricted to extraembryonic mesoderm of the visceral yolk sac.

Structure	Level	Pattern	Image	Note
TS10: yolk sac mesoderm	Present	Regionally restricted	3aD	Expression restricted to extraembryonic mesoderm of the visceral yolk sac.

Structure	Level	Pattern	Image	Note
TS11: yolk sac mesoderm	Strong	Widespread	3aE	Strongest expression in clusters of visceral mesoderm cells of the yolk sac, which constitute the precursors of the yolk sac blood islands. Expression throughout the visceral yolk sac mesoderm
TS11: embryo mesoderm	Present	Regionally restricted	3aE	Expression to a lesser extent in the anterior region of the intraembryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS11: yolk sac mesoderm	Present	Widespread	3aF	Expression throughout the visceral yolk sac mesoderm.
TS11: embryo mesoderm	Present	Regionally restricted	3aF	Expression to a lesser extent in the anterior region of the intraembryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS12: dorsal aorta	Present	Regionally restricted	3aG	Authors report expression around the dorsal aorta.
TS12: endocardial tube	Present		3aG
TS12: yolk sac mesenchyme	Present	Regionally restricted	3aG	Expression in endothelial cells lining the vascular plexus within the yolk sac blood islands.

Structure	Level	Pattern	Image	Note
TS12: blood island	Present	Regionally restricted	3aH	Expression endothelial cells lining the vascular plexus within the yolk sac blood islands.

Structure	Level	Pattern	Image	Note
TS13: endocardial tube	Present		3bA
TS13: head mesenchyme	Present		3bA
TS13: yolk sac	Present		3bA

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present	Regionally restricted	3bB	Expression in clusters of mesodermal cells in the head mesenchyme (presumably the angioblasts for the cephalic capillary plexus).

Structure	Level	Pattern	Image	Note
TS13: endocardial tube	Present		3bC

Structure	Level	Pattern	Image	Note
TS13: blood island	Present		3bD

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present	Regionally restricted	3bE	Expression localized to endothelial cells of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	3bF	Expression in clusters of mesodermal cells in the head mesenchyme (presumably the angioblasts for the cephalic capillary plexus).

Structure	Level	Pattern	Image	Note
TS15: endocardial tube	Present		3bG

Structure	Level	Pattern	Image	Note
TS15: yolk sac blood vessel	Present	Regionally restricted	3bH	No expression in the innermost, spherical primitive blood cells.