Gene Expression Data
Assay Details
Assay
|
Reference:
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J:14410
Iruela-Arispe ML, et al., Differential expression of thrombospondin 1, 2, and 3 during murine development. Dev Dyn. 1993 May;197(1):40-56
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5805879
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Gene symbol:
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Thbs3
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Gene name:
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thrombospondin 3
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Probe:
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probe E
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Probe preparation:
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Double stranded labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Multiple probes were used. Probe D (MGI:15598) or Probe F (MGI:16207) may also have been used.
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Results
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Specimen
6C:
embryonic day 18.5
(more )
Specimen
6C:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
3% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: long bone |
Present |
Regionally restricted |
6C
|
Expression in the early proliferative zone. Low levels of expression in osteoblasts associated with newly-deposited osteoid.
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Specimen
6F:
embryonic day 18.5
(more )
Specimen
6F:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: endochondral bone |
Present |
Regionally restricted |
6F
|
Expression in the early proliferative zone.
|
Specimen
6I:
embryonic day 17.5
(more )
Specimen
6I:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: vertebra |
Present |
Regionally restricted |
6I
|
Expression restricted to mature and fully differentiated chondrocytes.
|
Specimen
6L:
embryonic day 16.5
(more )
Specimen
6L:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: rib |
Present |
Regionally restricted |
6L
|
Expression restricted to mature and fully differentiated chondrocytes.
|
Specimen
6O:
embryonic day 17.5
(more )
Specimen
6O:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: tail |
Present |
Regionally restricted |
6O
|
Expression restricted to mature and fully differentiated chondrocytes.
|
Specimen
7C:
embryonic day 17.5
(more )
Specimen
7C:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
7F:
embryonic day 18.5
(more )
Specimen
7F:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8C:
postnatal newborn
(more )
Specimen
8C:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8F:
postnatal newborn
(more )
Specimen
8F:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8I:
postnatal newborn
(more )
Specimen
8I:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal newborn
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8L:
postnatal newborn
(more )
Specimen
8L:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal newborn
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8O:
postnatal newborn
(more )
Specimen
8O:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8R:
postnatal newborn
(more )
Specimen
8R:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
3% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|