Probe:	probe A
Probe preparation:	Double stranded labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Multiple probes were used. Authors specifically state that Probe A was used for specimens shown in Figure 2. For other specimens, Probe B (MGI:16204) may have been used.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Strong		2B , 2A

Structure	Level	Pattern	Image	Note
TS19: hematopoietic system	Present	Regionally restricted	2C	Expression in large circular cell in lumen of blood vessel appears to be of hematopoetic origin, tentatively identified as a circulating megakaryocyte.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present		2D
TS20: brain	Strong	Regionally restricted	2D	Abundant expression in neuronal cells adjacent to the lumen.

Structure	Level	Pattern	Image	Note
TS24: brain	Weak	Regionally restricted	2E	After day 13, significantly lower expression levels, mostly confined to connective tissue, blood vessels and choroid plexus.
TS24: choroid plexus	Present		2E	Expression in presumptive choroid plexus.

Structure	Level	Pattern	Image	Note
TS20: meninges	Present		2F

Structure	Level	Pattern	Image	Note
TS21: endocardial cushion	Present	Regionally restricted	2G	Authors report expression in cardiac cushions. Expression confined to mesenchyme of cardiac cushions.

Structure	Level	Pattern	Image	Note
TS22: endocardial cushion	Present	Regionally restricted	2H	Expression prevalent in cushion. Expression confined to mesenchyme of cardiac cushions.

Structure	Level	Pattern	Image	Note
TS24: parietal pericardium	Present		2I
TS24: visceral pericardium	Absent		2I

Structure	Level	Pattern	Image	Note
TS20: hematopoietic system	Present	Regionally restricted	2J	Expression in megakaryocytes in the liver.

Structure	Level	Pattern	Image	Note
TS25: hematopoietic system	Present	Regionally restricted	2K	Expression in megakaryocytes in the liver.

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present	Regionally restricted	2L	Expression concentrated at interface between cortical and medullary areas.

Structure	Level	Pattern	Image	Note
TS24: liver	Present		2M
TS24: metanephros	Present		2M
TS24: stomach muscularis externa	Present	Regionally restricted	2M	Expression in developing smooth muscle layer.

Structure	Level	Pattern	Image	Note
TS24: stomach muscularis externa	Present	Regionally restricted	2N	Expression in developing smooth muscle layer.

Structure	Level	Pattern	Image	Note
TS24: small intestine smooth muscle	Present		2O	Expression in the muscular layer.

Structure	Level	Pattern	Image	Note
TS24: vibrissa	Present	Regionally restricted	2P	Expression thoughout connective tissue and in the sheath that surrounds each vibrissa.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Weak	Widespread	2Q	Low levels of expression throughout the brain.
TS25: meninges	Strong		2Q

Structure	Level	Pattern	Image	Note
TS25: secondary palate	Strong		2R	Abundant expression in developing palate.
TS25: cochlea	Present	Regionally restricted	2R	Expression in cartilaginous tissues including the cochlea.
TS25: cochlea epithelium	Present		2R	Expression in neuroepithelium of cochlea.

Structure	Level	Pattern	Image	Note
TS26: long bone	Present	Regionally restricted	6A	Expression in nearly all chondrocytes. Expression in resting and hypertrophic zones. Strong expression in most osteoblasts associated with newly-deposited osteoid.

Structure	Level	Pattern	Image	Note
TS26: endochondral bone	Present	Regionally restricted	6D	Expression in resting and hypertrophic zones.

Structure	Level	Pattern	Image	Note
TS25: vertebra	Present	Regionally restricted	6G	Expression in nearly all chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: rib	Present	Regionally restricted	6J	Expression in nearly all chondrocytes.

Structure	Level	Pattern	Image	Note
TS25: tail	Present	Regionally restricted	6M	Expression in nearly all chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present		7A	Expression initially detected in lung at day 14.

Structure	Level	Pattern	Image	Note
TS23: bronchus epithelium	Present		7D	Expression restricted to columnar epithelium lining presumptive bronchial tree.

Structure	Level	Pattern	Image	Note
TS17: brain	Strong			High levels of expression in the brain as early as day 10.

Structure	Level	Pattern	Image	Note
TS19: heart	Present			Authors state that expression in the heart was coincidental with the initiation of septation (day 11). Expression first detected in cardiac endothelium.

Structure	Level	Pattern	Image	Note
TS20: brain	Strong	Regionally restricted		Abundant expression in neuroepithelial cells.

Structure	Level	Pattern	Image	Note
TS21: brain	Absent			Expression in the brain diminished abruptly and was absent by day 13.

Structure	Level	Pattern	Image	Note
TS24: bronchus epithelium	Present

Structure	Level	Pattern	Image	Note
TS26: bronchus epithelium	Present
TS26: meninges	Present

Structure	Level	Pattern	Image	Note
TS27: meninges	Present

Structure	Level	Pattern	Image	Note
TS27: brain	Present		8A

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	8D	Expression in a subset of neurons from the ventricular olfactory bulb.

Structure	Level	Pattern	Image	Note
TS27: accessory olfactory bulb	Absent		8G

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Absent		8J

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Weak		8M	Low levels of expression in the cerebellum.
TS27: choroid plexus	Present		8M

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Absent		8P