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Gene Expression Data
Assay Details
Assay
Reference: J:231834 Vanhoutteghem A, et al., The importance of basonuclin 2 in adult mice and its relation to basonuclin 1. Mech Dev. 2016 May;140:53-73
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5805472
Gene symbol: Bnc1
Gene name: basonuclin zinc finger protein 1
Antibody: BNC1 antibody
Detection system: Cy3
Results
Specimen 2A: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: surface epithelium of ovary Present Regionally restricted 2A Expression in nuclei of outer epithelial cells.
TS27: ovary Present Regionally restricted 2A Expression in a few intra-ovarian cells.
Specimen 2C/E: postnatal newborn (more )
Note: Double labeled: red - Bnc1; green - Sycp3 (assay MGI:5805476).
Structure Level Pattern Image Note
TS27: surface epithelium of ovary Present 2C , 2E
TS27: oocyte of primordial follicle Present Regionally restricted 2C , 2E Expression in inner ovarian cells (primordial oocytes). More abundant in leptotene than in zygotene oocytes.
Specimen 2F: postnatal day 60 (more )
Note: Triple labeled: red - Bnc1; red - Vasa (Ddx4 assay MGI:5805477); blue - DAPI.
Structure Level Pattern Image Note
TS28: surface epithelium of ovary Present Regionally restricted 2F Nuclear expression in epithelial cells.
TS28: oocyte of primordial follicle Absent 2F
Specimen 2G: postnatal day 60 (more )
Note: Triple labeled: red - Bnc1; red - Vasa (Ddx4 assay MGI:5805477); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of ovary Strong Regionally restricted 2G Abundant expression in nuclei of oocytes in follicles.
Specimen 3F/H: postnatal day 8 (more )
Note: Double labeled: red - Bnc1; green PLZF (Zbtb16 assay MGI:5805478).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 3F , 3H Expression in a substantial number of tubular cells, mostly undifferentiated spermatogonia.
TS28: spermatogonium Present Regionally restricted 3H , 3F Expression in undifferentiated and differentiating spermatogonia.
Specimen 3I: postnatal day 15 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Regionally restricted 3I Expression confined to spermatogonia resting on the basal membrane.
Specimen 3K/M: postnatal day 30 (more )
Note: Double labeled: red - Bnc1; green PLZF (Zbtb16 assay MGI:5805478).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 3K , 3M Expression in undifferentiated and differentiating spermatogonia. No expression in meiotic or postmeiotic cells.
Specimen 5I: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: stomach wall Present Regionally restricted 5I Expression in outer surface of stomach.
Specimen 5K: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: small intestine Present Regionally restricted 5K Expression localized in serosal coat of small intestine. No expression in smooth muscle layers.
Specimen 6B: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: dorsal root ganglion Present Regionally restricted 6B
Specimen 6I/J: postnatal newborn (more )
Note: Triple labeled: red - Bnc1; green - Bnc2 (assay MGI:5805469); blue - DAPI.
Structure Level Pattern Image Note
TS27: dorsal root ganglion Present Regionally restricted 6I , 6J Expression in neurons.
Specimen 11B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: palate epithelium Present Regionally restricted 11B In an anterior section, expression specifically in medial part of oral epithelium.`
Specimen 11C: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: palate epithelium Present Regionally restricted 11C In a more posterior section, expression in epithelial triangle at the midline, where palatal shelves have just fused.
Specimen 12H: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: palate epithelium Present Regionally restricted 12H Expression confined to epithelium on oral side.
Specimen S1I: postnatal newborn; Bnc2Gt(pU21)18Imeg/Bnc2Gt(pU21)18Imeg (more )
Structure Level Pattern Image Note
TS27: coat hair follicle Present S1I
TS27: epidermis Present S1I
Specimen S1J: postnatal newborn; Bnc2Gt(pU21)18Imeg/Bnc2Gt(pU21)18Imeg (more )
Structure Level Pattern Image Note
TS27: germ cell of testis Present Regionally restricted S1J Expression in testicular prospermatogonia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
MGI 6.23 		The Jackson Laboratory