Tg(tetO-TNF)15Wxlr
Transgene Detail
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| Symbol: |
Tg(tetO-TNF)15Wxlr |
| Name: |
transgene insertion 15, Viktor Wixler |
| MGI ID: |
MGI:5804529 |
| Synonyms: |
ihTNFtg, Tg_ihTNFalphaF15, Tg(tetO-TNF)15 |
| Transgene: |
Tg(tetO-TNF)15Wxlr Location: unknown
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| Alliance: |
Tg(tetO-TNF)15Wxlr page
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| Transgene Type: |
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Transgenic (Humanized sequence, Inducible, Inserted expressed sequence) |
| Inducer: |
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doxycycline/tetracycline |
| Mutation: |
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Insertion
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Tg(tetO-TNF)15Wxlr expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
TNF (7124) |
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Mutation details: This transgene contains the tetracycline-responsive P-tight promoter (from the plasmid pTRE-Tight, BD Biosciences Clontech), which comprises a modified Tet-responsive element containing seven direct repeats of a 36-bp sequence including the 19-bp tet operator sequence followed by a minimal cytomegalovirus (CMV) promoter, driving expression of a human tumor necrosis factor alpha cDNA whose 3' untranslated region (3'-UTR) has been replaced by the SV40 polyadenylation signal. This transgenic line was estimated by Southern blot analysis to carry 5 tandem copies of the transgene. No human TNFalpha is detected by enzyme-linked immunosorbent assay (ELISA) in sera of doxycycline-treated or untreated mice bearing this transgene alone.
(J:236003)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:236003 Retser E, et al., Doxycycline-induced expression of transgenic human tumor necrosis factor alpha in adult mice results in psoriasis-like arthritis. Arthritis Rheum. 2013 Sep;65(9):2290-300 |
| All: |
8 reference(s) |
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Analysis Tools
