Tcp1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:2891 Kubota H, et al., Structure and expression of the gene encoding mouse t-complex polypeptide (Tcp-1). Gene. 1992 Oct 21;120(2):207-15
Assay type:	Northern blot
MGI Accession ID:	MGI:5804202
Gene symbol:	Tcp1
Gene name:	t-complex protein 1

Probe:	pT1b11
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

Image: 5B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
Testis	P adult	TS28: testis	Present	10 µg; total RNA	involves: 129		Male
Testis (1/10)	P adult	TS28: testis	Present	1 µg; total RNA	involves: 129		Male
Testis (1/100)	P adult	TS28: testis	Ambiguous	0.1 µg; total RNA	involves: 129		Male
Uterus	P adult	TS28: uterus	Ambiguous	10 µg; total RNA	involves: 129		Female
7.5-Day Embryo (+Decidua)	E7.5	TS11: conceptus TS28: decidua	Present	10 µg; total RNA	involves: 129		Not Specified
9.5-Day Embryo (+Decidua)	E9.5	TS15: conceptus TS28: decidua	Present (a)	10 µg; total RNA	involves: 129		Not Specified
11.5-Day Embryo	E11.5	TS19: embryo	Present (b)	10 µg; total RNA	involves: 129		Not Specified
13.5-Day Embryo	E13.5	TS21: embryo	Present (c)	10 µg; total RNA	involves: 129		Not Specified
15.5-Day Embryo	E15.5	TS23: embryo	Present (c)	10 µg; total RNA	involves: 129		Not Specified

Notes:
(a) The transcript level decreased rapidly between E7.5-9.5 (70% decrease).
(b) E11.5 contain transcript at 30% of the level in the testis.
(c) Between E11.5-15.5 the transcript level continued to decrease (40-60% decrease per two days).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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