Gene Expression Data
Assay Details
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Assay
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Reference:
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J:2891
Kubota H, et al., Structure and expression of the gene encoding mouse t-complex polypeptide (Tcp-1). Gene. 1992 Oct 21;120(2):207-15
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Assay type:
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Northern blot
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MGI Accession ID:
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MGI:5804196
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Gene symbol:
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Tcp1
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Gene name:
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t-complex protein 1
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Probe:
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pT1b11
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Probe preparation:
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Double stranded labelled with P32 DNA
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Visualized with:
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Autoradiography
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Assay notes:
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Authors indicate there were three minor transcript species (2.4, 3.7 and over 9kb) seen in addition to the major 2.0kb species. The 2.4 and 3.7 transcripts might
use different polyadenylation sites from the 2.0-kb transcripts
or, alternatively, encode TCP-l-like proteins. The transcripts larger than 9 kb might be the
hnRNA of Tcp-1 or, again, might encode one of these
TCP-l-like proteins. Alternatively, it is possible that the
three minor RNA species were partially denatured or incompletely
spliced.
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Results
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Image: 5A
| P adult
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TS28: testis
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Strong
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10 µg; total RNA |
involves: 129 |
| Male |
| P adult
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TS28: testis
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Present
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1 µg; total RNA |
involves: 129 |
| Male |
| P adult
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TS28: testis
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Present
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0.1 µg; total RNA |
involves: 129 |
| Male |
| P adult
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TS28: bone marrow
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Present
(a)
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10 µg; total RNA |
involves: 129 |
| Not Specified |
| P w 4
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TS28: thymus
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Present
(a)
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10 µg; total RNA |
involves: 129 |
| Not Specified |
| P adult
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TS28: heart
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Present
(b)
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10 µg; total RNA |
involves: 129 |
| Not Specified |
| P adult
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TS28: brain
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Present
(b)
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10 µg; total RNA |
involves: 129 |
| Not Specified |
| P adult
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TS28: lung
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Present
(c)
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10 µg; total RNA |
involves: 129 |
| Not Specified |
Notes:
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Analysis Tools