Gene Expression Data
Assay Details
Assay
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Reference:
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J:72525
Schubert FR, et al., Early mesodermal phenotypes in splotch suggest a role for Pax3 in the formation of epithelial somites. Dev Dyn. 2001 Nov;222(3):506-21
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5803700
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Results
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Specimen
4A:
embryonic day 12.0
(more )
Specimen
4A:
(close )
Genetic Background:
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involves: C57BL/6 * DBA/2 * NMRI
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS20: sclerotome |
Strong |
Regionally restricted |
4A
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Expression was strong in the caudal halves of the lumbar sclerotomes, as well as in the centers of the sclerotomes.
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Specimen
4B:
(close )
Genetic Background:
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involves: 101/Rl * C3H/Rl * NMRI
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Mutant Allele(s):
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Dll3pu/Dll3pu
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
|
whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS20: sclerotome |
Strong |
Regionally restricted |
4B
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Signals were fused. There was an irregular array of elevated signals.
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Specimen
4C:
embryonic day 12.0; rh/ rh
(more )
Specimen
4C:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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rh/rh
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Age:
|
embryonic day 12.0
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: sclerotome |
Present |
Regionally restricted |
4C
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Signal was fused.
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Specimen
4D:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: sclerotome |
Present |
Regionally restricted |
4D
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The ventromedial to dorsolateral extent of expression was normal. However, the three sclerotomes indicated display irregular and fused signals with altered rostrocaudal pattern. Strong signal in the rostral sclerotome halves, lateral to the negative spots. In the caudal sclerotome halves and ventromedially levels of expression are reduced.
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Specimen
4E:
embryonic day 10.5
(more )
Specimen
4E:
(close )
Genetic Background:
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involves: C57BL/6 * DBA/2 * NMRI
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: sclerotome |
Present |
Regionally restricted |
4E
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Expression was highest in the caudal sclerotome halves.
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TS17: spinal nerve |
Absent |
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4E
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Expression was negative in the spinal nerve in the lateral territory of the rostral sclerotome half.
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Specimen
4F:
(close )
Genetic Background:
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involves: 101/Rl * C3H/Rl * NMRI
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Mutant Allele(s):
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Dll3pu/Dll3pu
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: sclerotome |
Present |
Regionally restricted |
4F
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Signals were fused. Lighter and darker staining appears at irregular intervals.
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Specimen
4G:
embryonic day 10.5; rh/ rh
(more )
Specimen
4G:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
|
rh/rh
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Age:
|
embryonic day 10.5
|
Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: sclerotome |
Present |
Regionally restricted |
4G
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Signals were fused with ill-defined rostrocaudal pattern.
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Specimen
4H:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
|
Pax3Sp/Pax3Sp
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: sclerotome |
Present |
Regionally restricted |
4H
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The rostrocaudal pattern of expression was altered. The ventromedial to dorsolateral extent was not changed. Sclerotomes exhibited fused signals and an erroneous pattern of up-regulation.
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