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Gene Expression Data
Assay Details
Assay
Reference: J:229524 Robert T, et al., The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation. Science. 2016 Feb 26;351(6276):943-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5800422
Gene symbol: H1f6
Gene name: H1.6 linker histone, cluster member
Antibody: Anti-H1t
Detection system: Secondary antibody
Results
Specimen S18 +/+ Leptotene: postnatal day 30 (more )
Note: Double labeled: green - H1f6; red - Sycp3 (assay MGI:5800404). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Absent S18+/+ Leptotene H1t , S18+/+ Leptotene Merge
Specimen S18 +/+ Zygotene: postnatal day 30 (more )
Note: Double labeled: green - H1f6; red - Sycp3 (assay MGI:5800404). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Absent S18+/+ Zygotene Merge , S18+/+ Zygotene H1t
Specimen S18 +/+ Pachytene: postnatal day 30 (more )
Note: Double labeled: green - H1f6; red - Sycp3 (assay MGI:5800404). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S18+/+ Pachytene H1t , S18+/+ Pachytene Merge Expression was detected from mid-pachytene.
Specimen S18 -/- Leptotene: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: green - H1f6; red - Sycp3 (assay MGI:5800404). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Absent S18-/- Leptotene Merge , S18-/- Leptotene H1t
Specimen S18 -/- Zygo Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: green - H1f6; red - Sycp3 (assay MGI:5800404). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Ambiguous S18-/- Zygo Like Merge , S18-/- Zygo Like H1t Mutant spermatocytes were devoid of or showed only low expression.
Specimen S18 -/- Zygo. Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: green - H1f6; red - Sycp3 (assay MGI:5800404). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Ambiguous S18-/- Zygo. Like Merge , S18-/- Zygo. Like H1t Mutant spermatocytes were devoid of or showed only low expression.
Specimen S18 -/- Zygo. Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: green - H1f6; red - Sycp3 (assay MGI:5800404). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Ambiguous S18-/- Zygo. Like H1t , S18-/- Zygo. Like Merge Mutant spermatocytes were devoid of or showed only low expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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