Sycp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:229524 Robert T, et al., The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation. Science. 2016 Feb 26;351(6276):943-9
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5800309
Gene symbol:	Sycp3
Gene name:	synaptonemal complex protein 3

Antibody:	Anti-Sycp3
Detection system:	Secondary antibody

Results

Specimen 5A +/+ Leptotene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Rpa1 (assay MGI:5800304). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5A +/+ Leptotene Sycp3 , 5A +/+ Leptotene Merge

Specimen 5A +/+ Zygotene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Rpa1 (assay MGI:5800304). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5A +/+ Zygotene Merge , 5A +/+ Zygotene Sycp3

Specimen 5A +/+ Pachytene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Rpa1 (assay MGI:5800304). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5A +/+ Pachytene Merge , 5A +/+ Pachytene Sycp3

Specimen 5A -/- Leptotene: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Rpa1 (assay MGI:5800304). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5A -/- Leptotene Sycp3 , 5A -/- Leptotene Merge

Specimen 5A -/- Zygotene Like: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Rpa1 (assay MGI:5800304). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5A -/- Zygotene like Merge upper , 5A -/- Zygotene like Sycp3 upper

Specimen 5A -/- Zygotene Like: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Rpa1 (assay MGI:5800304). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5A -/- Zygotene like Sycp3 lower , 5A -/- Zygotene like Merge lower

Specimen 5B +/+ Leptotene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5B +/+ Leptotene Sycp3 , 5B +/+ Leptotene Merge

Specimen 5B +/+ Zygotene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5B +/+ Zygotene Merge , 5B +/+ Zygotene Sycp3

Specimen 5B +/+ Pachytene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5B +/+ Pachytene Merge , 5B +/+ Pachytene Sycp3

Specimen 5B -/- Leptotene: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5B -/- Leptotene Merge , 5B -/- Leptotene Sycp3

Specimen 5B -/- Zygotene Like: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5B -/- Zygotene like Merge upper , 5B -/- Zygotene like Sycp3 upper

Specimen 5B -/- Zygotene Like: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5B -/- Zygotene like Sycp3 lower , 5B -/- Zygotene like Merge lower

Specimen 5C +/+ Leptotene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C +/+ Leptotene Sycp3 , 5C +/+ Leptotene Merge

Specimen 5C +/+ Zygotene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C +/+ Zygotene Sycp3 , 5C +/+ Zygotene Merge

Specimen 5C +/+ Pachytene: postnatal day 30 (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C +/+ Pachytene Merge , 5C +/+ Pachytene Sycp3

Specimen 5C -/- Leptotene: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C -/- Leptotene Merge , 5C -/- Leptotene Sycp3

Specimen 5C -/- Zygotene Like: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C -/- Zygotene like Merge upper , 5C -/- Zygotene like Sycp3 upper

Specimen 5C -/- Zygotene Like: postnatal day 30; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C -/- Zygotene like Sycp3 lower , 5C -/- Zygotene like Merge lower

Specimen S16A -/- Zygotene Like: postnatal month 8; Top6bl^em2Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S16A Merge , S16A Sycp3

Specimen S17B +/+ Leptotene: postnatal month 8 (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17B_+/+ Leptotene Sycp3 , S17B_+/+ Leptotene merge

Specimen S17B +/+ Zygotene: postnatal month 8 (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17B_+/+ Zygotene Sycp3 , S17B_+/+ Zygotene merge

Specimen S17B +/+ Pachytene: postnatal month 8 (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17B_+/+ Pachytene Sycp3 , S17B_+/+ Pachytene merge

Specimen S17B -/- Leptotene: postnatal month 8; Top6bl^em1Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17B_-/- Leptotene Sycp3 , S17B_-/- Leptotene merge

Specimen S17B -/- Zygo. Like: postnatal month 8; Top6bl^em1Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17B_-/- Zygo. Like Sycp3 , S17B_-/- Zygo. Like merge

Specimen S17B -/- Zygo Like: postnatal month 8; Top6bl^em1Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Sycp1 (assay MGI:5800305). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17B_-/- Zygo Like merge , S17B_-/- Zygo Like Sycp3

Specimen S17C +/+ Leptotene: postnatal month 8 (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17C_+/+ Leptotene Merge , S17C_+/+ Leptotene Sycp3

Specimen S17C +/+ Zygotene: postnatal month 8 (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17C_+/+ Zygotene Sycp3 , S17C_+/+ Zygotene Merge

Specimen S17C +/+ Pachytene: postnatal month 8 (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17C_+/+ Pachytene Sycp3 , S17C_+/+ Pachytene Merge

Specimen S17C -/- Leptotene: postnatal month 8; Top6bl^em1Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17C_-/- Leptotene Merge , S17C_-/- Leptotene Sycp3

Specimen S17C -/- Zygo. Like: postnatal month 8; Top6bl^em1Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17C_-/- Zygo. Like Merge , S17C_-/- Zygo. Like Sycp3

Specimen S17C -/- Zygo Like: postnatal month 8; Top6bl^em1Arte/Top6bl^em2Arte (more

)
Note: Double labeled: green - Sycp3; red - Mei4 (assay MGI:5800306). Meiotic nuclear spread from testis.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S17C_-/- Zygo Like Merge , S17C_-/- Zygo Like Sycp3

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