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Gene Expression Data
Assay Details
Assay
Reference: J:229524 Robert T, et al., The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation. Science. 2016 Feb 26;351(6276):943-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5800306
Gene symbol: Mei4
Gene name: meiotic double-stranded break formation protein 4
Antibody: Anti-Mei4
Detection system: Secondary antibody
Results
Specimen 5C +/+ Leptotene: postnatal day 30 (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5C +/+ Leptotene Mei4 , 5C +/+ Leptotene Merge
Specimen 5C +/+ Zygotene: postnatal day 30 (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5C +/+ Zygotene Mei4 , 5C +/+ Zygotene Merge
Specimen 5C +/+ Pachytene: postnatal day 30 (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Absent 5C +/+ Pachytene Mei4 , 5C +/+ Pachytene Merge
Specimen 5C -/- Leptotene: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5C -/- Leptotene Mei4 , 5C -/- Leptotene Merge Localization was similar in mutant and wild type at leptotene.
Specimen 5C -/- Zygotene Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5C -/- Zygotene like Mei4 upper , 5C -/- Zygotene like Merge upper Expression was persistent in mutant zygotene-like stages.
Specimen 5C -/- Zygotene Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5C -/- Zygotene like Merge lower , 5C -/- Zygotene like Mei4 lower Expression was persistent in mutant zygotene-like stages.
Specimen S17C +/+ Leptotene: postnatal month 8 (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17C_+/+ Leptotene Merge , S17C_+/+ Leptotene Mei4
Specimen S17C +/+ Zygotene: postnatal month 8 (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17C_+/+ Zygotene Merge , S17C_+/+ Zygotene Mei4
Specimen S17C +/+ Pachytene: postnatal month 8 (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Absent S17C_+/+ Pachytene Merge , S17C_+/+ Pachytene Mei4
Specimen S17C -/- Leptotene: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17C_-/- Leptotene Mei4 , S17C_-/- Leptotene Merge
Specimen S17C -/- Zygo. Like: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17C_-/- Zygo. Like Mei4 , S17C_-/- Zygo. Like Merge Expression was persistent in mutant zygotene-like stages.
Specimen S17C -/- Zygo Like: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Double labeled: red - Mei4; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17C_-/- Zygo Like Mei4 , S17C_-/- Zygo Like Merge Expression was persistent in mutant zygotene-like stages.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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