Gene Expression Data
Assay Details
Assay
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Reference:
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J:135516
Wansbury O, et al., Dynamic expression of Erbb pathway members during early mammary gland morphogenesis. J Invest Dermatol. 2008 Apr;128(4):1009-21
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5800279
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Gene symbol:
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Erbb2
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Gene name:
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erb-b2 receptor tyrosine kinase 2
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Antibody:
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anti-Erbb2 (C18; sc-284)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixative was either: 4% paraformaldehyde, formalin, or Carnoy's fixative.
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Results
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Specimen
3c:
embryonic day 11.0
(more )
Specimen
3c:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3d:
embryonic day 12.0
(more )
Specimen
3d:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: mammary bud |
Present |
|
3d
|
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TS20: epidermis |
Present |
Regionally restricted |
3d
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Expression is in the surface epithelium adjacent to the mammary bud.
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Specimen
3e:
embryonic day 13.5
(more )
Specimen
3e:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 13.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
|
Paraffin
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Specimen
3f:
embryonic day 14.5
(more )
Specimen
3f:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: epidermis |
Moderate |
Regionally restricted |
3f
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Expression is in the surface epithelium adjacent to the mammary bud.
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TS22: mammary bud |
Moderate |
|
3f
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Moderate expression in the neck of the mammary bud and weak in the mature mammary bud.
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TS22: mammary mesenchyme |
Present |
Regionally restricted |
3f
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Expression is in the mesenchyme in the vicinity of the future site of the fat pad.
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TS22: connective tissue |
Present |
Regionally restricted |
3f
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Expression is at the site of the future fat pad.
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Specimen
S2:
embryonic day 12.5
(more )
Specimen
S2:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
|
Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
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