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Gene Expression Data
Assay Details
Assay
Reference: J:135516 Wansbury O, et al., Dynamic expression of Erbb pathway members during early mammary gland morphogenesis. J Invest Dermatol. 2008 Apr;128(4):1009-21
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5800274
Gene symbol: Nrg3
Gene name: neuregulin 3
Antibody: Nrg3 EGF-like domain antibody
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: Fixative was either: 4% paraformaldehyde, formalin, or Carnoy's fixative. Three different polyclonal antibodies may have been used: Nrg3 (P-18) antibody (MGI:5800276), Nrg3 (D-19) antibody (MGI:5800277, or Nrg3 antibody (MGI:5800291).
Results
Specimen 8f: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: mammary placode Present 8f
TS19: surface ectoderm Present Regionally restricted 8f Expression is in the epithelium adjacent to the mammary placode.
Specimen 8g: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: mammary bud Present 8g
TS20: epidermis Present Regionally restricted 8g Expression is in the epithelium adjacent to the mammary bud.
Specimen 8h: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: mammary bud Moderate 8h
TS21: connective tissue Present Regionally restricted 8h Expression is at the site of the future mammary fat pad.
Specimen 8i: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: mammary bud Weak 8i Expression is higher in the neck of the bud.
TS22: epidermis Present Regionally restricted 8i Expression is in the epithelium adjacent to the mammary bud.
TS22: connective tissue Present Regionally restricted 8i Expression is at the site of the future mammary fat pad.
Specimen S2: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: mammary bud Present S2_Nrg3
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory