Gene Expression Data
Assay Details
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Assay
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Reference:
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J:135516
Wansbury O, et al., Dynamic expression of Erbb pathway members during early mammary gland morphogenesis. J Invest Dermatol. 2008 Apr;128(4):1009-21
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5800273
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Gene symbol:
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Nrg4
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Gene name:
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neuregulin 4
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Probe:
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Nrg4 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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Vibratome sections.
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Results
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Specimen
9a:
embryonic day 11.0
(more  )
Specimen
9a:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: somite |
Present |
Regionally restricted |
9b
,
9a
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Expression is in the epaxial domain.
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| TS18: trunk mesenchyme |
Weak |
Regionally restricted |
9b
,
9a
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Expression is in the lateral plate mesoderm ventral to the future site of the mammary placode.
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| TS18: surface ectoderm |
Absent |
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9b
,
9a
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Specimen
9c:
embryonic day 11.25
(more )
Specimen
9c:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.25
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: somite |
Present |
Regionally restricted |
9d
,
9c
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Expression is in the epaxial, central, and hypaxial domains.
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| TS19: mammary placode |
Present |
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9d
,
9c
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| TS19: trunk mesenchyme |
Present |
Regionally restricted |
9c
,
9d
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Expression is in the dermal mesenchyme underlying the mammary placode.
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Specimen
9e:
embryonic day 11.5
(more )
Specimen
9e:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: somite |
Present |
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9e
,
9f
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| TS19: mammary placode |
Present |
Regionally restricted |
9f
,
9e
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Expression is in the epithelium at the dorsal and ventral edges of the mammary placode.
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| TS19: trunk mesenchyme |
Present |
Regionally restricted |
9e
,
9f
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Expression is in the dermal mesenchyme underlying the epithelium directly adjacent to but not under the mammary placode.
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| TS19: surface ectoderm |
Present |
Regionally restricted |
9e
,
9f
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Expression is in the epithelium along the flank.
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Analysis Tools