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Gene Expression Data
Assay Details
Assay
Reference: J:218065 Hayata T, et al., Dullard/Ctdnep1 Regulates Endochondral Ossification via Suppression of TGF-beta Signaling. J Bone Miner Res. 2015 Feb;30(2):318-29
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5796787
Gene symbol: Smad3
Gene name: SMAD family member 3
Antibody: anti-phospho-Smad2/3 (9510)
Assay notes: The antibody recognizes the phosphorylated form of the protein. Both Smad2 and Smad3 are annotated. A TSA-biotin system was used for detection.
Results
Specimen 5 Digit WT: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: digit mesenchyme Present Regionally restricted 5_Digit WT Expression is in the perichondrium.
Specimen 5 Digit KO: embryonic day 13.5; Ctdnep1tm3Ryn/Ctdnep1tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: digit mesenchyme Very strong Regionally restricted 5_Digit KO Expression is in the perichondrium.
Specimen 5 Humerus WT: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: humerus Present Regionally restricted 5_Humerus WT Expression is in the prehypertrophic and hypertrophic chondrocytes in the growth plate.
Specimen 5 Humerus KO: embryonic day 15.5; Ctdnep1tm3Ryn/Ctdnep1tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: humerus Very strong Regionally restricted 5_Humerus KO Expression is in the prehypertrophic and hypertrophic chondrocytes in the growth plate.
Specimen S10 Sternum WT: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: sternum Present S10_Sternum WT
Specimen S10 Sternum KO: embryonic day 15.5; Ctdnep1tm3Ryn/Ctdnep1tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: sternum Present S10_Sternum KO Expression in this mutant is the same as in wild-type embryo.
Specimen S10 Metacarpal WT: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: metacarpal bone Present S10_Metacarpal WT
Specimen S10 Metacarpal KO: embryonic day 15.5; Ctdnep1tm3Ryn/Ctdnep1tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: metacarpal bone Present S10_Metacarpal KO Expression in this mutant is the same as in wild-type embryo.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory