Nrg3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:100495 Howard B, et al., Identification of the scaramanga gene implicates Neuregulin3 in mammary gland specification. Genes Dev. 2005 Sep 1;19(17):2078-90
Assay type:	RT-PCR
MGI Accession ID:	MGI:5796683
Gene symbol:	Nrg3
Gene name:	neuregulin 3

Probe:	Nrg3-pC, Nrg3-pD
Assay notes:	Tissues were microdissected from the presumptive mammary region spanning the level of somites 11-24 where buds 2, 3 and 4 will form.

Results

Image: 5B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
A/J LPM 43-45 somite stage	E11.0 (a)	TS18: mesenchyme derived from lateral plate	Weak (b)	5 µg; total RNA	A/J	Nrg3^ska/Nrg3^ska	Not Specified	43-45 somites
B6 LPM 43-45 somite stage	E11.0 (a)	TS18: mesenchyme derived from lateral plate	Present	5 µg; total RNA	C57BL/6		Not Specified	43-45 somites
A/J LPM 46-48 somite stage	E11.5 (a)	TS19: mesenchyme derived from lateral plate	Weak (c)	5 µg; total RNA	A/J	Nrg3^ska/Nrg3^ska	Not Specified	46-48 somites
B6 LPM 46-48 somite stage	E11.5 (a)	TS19: mesenchyme derived from lateral plate	Present	5 µg; total RNA	C57BL/6		Not Specified	46-48 somites

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Authors report a 5.0-fold reduction in expression levels compared with tissue from B6 embryos.
(c) Authors report a 1.7-fold reduction in expression levels compared with B6 embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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