About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:205532 Wang S, et al., Transient activation of autophagy via Sox2-mediated suppression of mTOR is an important early step in reprogramming to pluripotency. Cell Stem Cell. 2013 Nov 7;13(5):617-25
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5796604
Gene symbol: Mtor
Gene name: mechanistic target of rapamycin kinase
Antibody: anti-mTOR (7C10) Rabbit mAb
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: Using another antibody (MGI:5796586) received similar results.
Results
Specimen 2D 1 cell: embryonic day 0.5 (more )
Note: 1-cell stage
Structure Level Pattern Image Note
TS1: 1-cell stage conceptus Present 2D mTOR upper , 2D merge upper
Specimen 2D 2 cell: embryonic day 1.5 (more )
Note: 2-cell stage
Structure Level Pattern Image Note
TS2: 2-cell stage conceptus Present 2D mTOR middle , 2D merge middle
Specimen 2D 4-8 cell: embryonic day 2.5 (more )
Note: 4-8-cell stage
Structure Level Pattern Image Note
TS3: 4-8 cell stage conceptus Ambiguous 2D mTOR bottom , 2D merge bottom Downregulated expression.
Specimen 2G Sox+/+: embryonic day 2.5 (more )
Note: 4-8-cell stage
Structure Level Pattern Image Note
TS3: 4-8 cell stage conceptus Ambiguous 2G mTOR upper , 2G merge upper Downregulated expression.
Specimen 2G Sox-/-: embryonic day 2.5; Sox2tm1.2Lan/Sox2tm1.2Lan (more )
Note: 4-8-cell stage
Structure Level Pattern Image Note
TS3: 4-8 cell stage conceptus Present 2G mTOR lower , 2G merge lower Expression was not reduced.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory