Il7 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:189751 Hara T, et al., Identification of IL-7-producing cells in primary and secondary lymphoid organs using IL-7-GFP knock-in mice. J Immunol. 2012 Aug 15;189(4):1577-84
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5796358
Gene symbol:	Il7
Gene name:	interleukin 7

Results

Specimen 2A left column: postnatal week 7; Il7^tm1.1Iku/Il7⁺ (more

)
Note: Double labeled: green - Il7 reporter; blue - Enpep (assay MGI:5796395).

Structure	Level	Pattern	Image	Note
TS28: thymus	Present		2A middle GFP , 2A left GFP Ly51 , 2A left GFP , 2A left HM	Expression is in thymic stromal cells.

Specimen 2A middle column: postnatal week 7; Il7^tm1.1Iku/Il7⁺ (more

)
Note: Double labeled: green - Il7 reporter; blue - Enpep (assay MGI:5796395).

Structure	Level	Pattern	Image	Note
TS28: thymus cortex	Present		2A middle GFP Ly51 , 2A middle GFP , 2A middle HM

Specimen 2A right column: postnatal week 7; Il7^tm1.1Iku/Il7⁺ (more

)
Note: Double labeled: green - Il7 reporter; blue - Enpep (assay MGI:5796395).

Structure	Level	Pattern	Image	Note
TS28: thymus cortex	Present		2A right GFP , 2A right GFP Ly51 , 2A right HM
TS28: thymus medullary core	Present		2A right GFP , 2A right GFP Ly51 , 2A right HM

Specimen 2C top: embryonic day 16.5; Il7^tm1.1Iku/Il7⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: thymus	Present		2C GFP

Specimen 2C bottom: embryonic day 16.5; Il7^tm1.1Iku/Il7⁺ (more

)
Note: Double labeled: green - Il7 reporter; red - Epcam (assay MGI:5796512).

Structure	Level	Pattern	Image	Note
TS24: thymus	Present		2C GFP EpCAM GFP-KI

Specimen 4B c KI: postnatal; Il7^tm1.1Iku/Il7⁺ (more

)
Note: Double labeled: green - Il7 reporter; red - Lyve1 (assay MGI:5804001).

Structure	Level	Pattern	Image	Note
TS28: lymph node	Present		4Bc GFP , 4Bc GFP Lyve1 , 4Bc HM GFP , 4Bc HM GFP Lyve1

Specimen 4E: postnatal; Il7^tm1.1Iku/Il7⁺ (more

)
Note: Double labeled: green - Il7 reporter; red - Lyve1 (assay MGI:5804001).

Structure	Level	Pattern	Image	Note
TS28: dermis	Present		4E IL-7-GFP-KI
TS28: ear skin	Present		4E IL-7-GFP-KI

Specimen 6A LI top: postnatal; Il7^tm1.1Iku/Il7⁺ (more

)
Note: Triple labeled: green - Il7 reporter; red - Itgam (assay MGI:5804009); blue - Cd4 (assay MGI:5804013).

Structure	Level	Pattern	Image	Note
TS28: large intestine	Present		6A GFP CD4 Mac1 control

Specimen 6A LI bottom: postnatal; Il7^tm1.1Iku/Il7⁺ (more

)
Note: Triple labeled: green - Il7 reporter; red - Itgax (assay MGI:5804010); blue - Cd4 (assay MGI:5804013).

Structure	Level	Pattern	Image	Note
TS28: large intestine	Present		6A GFP CD4 CD11c control

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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