Gene Expression Data
Assay Details
Assay
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Reference:
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J:230806
Arbi M, et al., GemC1 controls multiciliogenesis in the airway epithelium. EMBO Rep. 2016 Mar;17(3):400-13
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5796330
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Gene symbol:
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Mcidas
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Gene name:
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multiciliate differentiation and DNA synthesis associated cell cycle protein
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Probe:
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McIdas cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 10.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: brain |
Present |
Regionally restricted |
1A McIdas bottom left
,
1A McIdas bottom right
,
1A McIdas top
|
Expression was detected in the developing choroid plexus epithelium of all ventricles.
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Specimen
1B top:
embryonic day 15.5
(more )
Specimen
1B top:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: respiratory system epithelium |
Present |
Regionally restricted |
1B McIdas top
|
Expression was in the respiratory epithelium of the nasal cavity.
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Specimen
1B middle:
embryonic day 15.5
(more )
Specimen
1B middle:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: respiratory system epithelium |
Present |
Regionally restricted |
1B McIdas middle
|
Expression was in the respiratory epithelium of the oral cavity.
|
Specimen
1B bottom:
embryonic day 15.5
(more )
Specimen
1B bottom:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lobar bronchus epithelium |
Present |
Regionally restricted |
1B McIdas bottom
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Expression was detected in the upper bronchial tree in the lungs.
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Specimen
EV1C:
embryonic day 12.5
(more )
Specimen
EV1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: choroid plexus |
Present |
Regionally restricted |
EV1C"
,
EV1C'
,
EV1C
|
Expression in the developing choroid plexus epithelium was reduced and gradually confined to the base of the epithelium.
|
TS20: lateral ventricle choroid plexus |
Present |
Regionally restricted |
EV1C
,
EV1C"
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Expression in the developing choroid plexus epithelium was reduced and gradually confined to the base of the epithelium.
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Specimen
EV1D:
embryonic day 12.5
(more )
Specimen
EV1D:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: 4th ventricle choroid plexus |
Present |
Regionally restricted |
EV1D
,
EV1D'
|
Expression in the developing choroid plexus epithelium was reduced and gradually confined to the base of the epithelium.
|
Specimen
EV2C:
embryonic day 14.5
(more )
Specimen
EV2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: choroid plexus |
Present |
Regionally restricted |
EV2C'
,
EV2C
,
EV2C"
|
Expression in the developing choroid plexus epithelium was reduced and gradually confined to the base of the epithelium.
|
TS22: lateral ventricle choroid plexus |
Present |
Regionally restricted |
EV2C
,
EV2C'
|
Expression in the developing choroid plexus epithelium was reduced and gradually confined to the base of the epithelium.
|
Specimen
EV2D:
embryonic day 14.5
(more )
Specimen
EV2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: 4th ventricle choroid plexus |
Present |
Regionally restricted |
EV2D
|
Expression in the developing choroid plexus epithelium was reduced and gradually confined to the base of the epithelium.
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Specimen
EV3B:
embryonic day 18.5
(more )
Note: Double labeled: black - Mcidas; green - Foxj1 (assay MGI:5796477).
Specimen
EV3B:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: black - Mcidas; green - Foxj1 (assay MGI:5796477).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
Present |
Regionally restricted |
EV3B"
,
EV3B
,
EV3B'
|
Expression was detected in the distal airway and co-expressed Foxj1.
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