Probe:	pN
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The observed hybridization signal using this probe should reflect the expression patterns of 3 isoforms Oct2a, Oct2b and Oct2c, but not Octd.

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Strong		4B
TS20: diencephalon	Strong		4B
TS20: metencephalon	Strong		4B
TS20: medulla oblongata	Strong	Regionally restricted	4B	Expression was detected in the pontine flexurae.
TS20: midbrain roof plate	Strong	Regionally restricted	4B	Expression was confined to the most superficial cells layers.
TS20: brain	Strong	Clusters	4B	Several clusters of strongly expressing cells were detected in the areas where the optic chiasm, mammillary bodies, and neural hypophysis will later appear.
TS20: telencephalon	Absent		4B

Structure	Level	Pattern	Image	Note
TS20: spinal ganglion	Strong		4E

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Strong	Regionally restricted	4H	Expression was strong in the intermediate zone (mantle layer) mainly in the ventral part of the developing neural tube.

Structure	Level	Pattern	Image	Note
TS24: nasal epithelium	Weak	Regionally restricted	4K	Expression was detected in the nasal neuroepithelium.

Structure	Level	Pattern	Image	Note
TS28: accessory olfactory bulb mitral cell layer	Strong		5B

Structure	Level	Pattern	Image	Note
TS28: olfactory tract	Strong
TS28: olfactory tubercle	Strong
TS28: cerebral internal capsule	Absent
TS28: corpus callosum	Absent
TS28: optic tract	Absent

Structure	Level	Pattern	Image	Note
TS28: frontal cortex	Strong		5E
TS28: occipital cortex	Weak		5E	Expression was decreased in the parietal and occipital cortical regions.
TS28: parietal cortex	Weak		5E	Expression was decreased in the parietal and occipital cortical regions.
TS28: cortical layer II	Present		5E
TS28: cortical layer III	Present		5E
TS28: cortical layer IV	Present		5E

Structure	Level	Pattern	Image	Note
TS28: insular cortex	Present		5H
TS28: piriform cortex	Present		5H

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present		5K
TS28: CA2 pyramidal cell layer	Present		5K
TS28: CA3 pyramidal cell layer	Present		5K
TS28: hippocampus CA4	Present		5K	Expression was detected in the pyramidal cell layer.
TS28: dentate gyrus granule cell layer	Absent		5K

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		5N
TS28: cerebellum molecular layer	Moderate		5N

Structure	Level	Pattern	Image	Note
TS28: testis	Trace		7C