Tg(Kap)L49Msgr
Transgene Detail
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| Symbol: |
Tg(Kap)L49Msgr |
| Name: |
transgene insertion L49, Anna Meseguer |
| MGI ID: |
MGI:5792809 |
| Synonyms: |
KAP Tg, L-49, L49, pKAP2-KAP |
| Transgene: |
Tg(Kap)L49Msgr Location: unknown
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| Alliance: |
Tg(Kap)L49Msgr page
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| Strain of Origin: |
(C57BL/6 x SJL)F2
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Kap)L49Msgr expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Kap (MGI:96653) |
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Mutation details: In male mice with this transgene, expression of cDNA encoding the mouse kidney androgen regulated protein (KAP) is driven by the mouse Kap promoter and the distal regulatory sequences of the human angiotensinogen gene (AGT), which together confer androgen responsiveness. The Kap cDNA was cloned downstream of the Kap promoter (nucleotides -1542 through -1 relative to the transcription initiation site) into the NotI site of the pKAP2 vector. This is followed by the last 42 bp of human AGT exon 2 through exon 5, which lost their coding potential owing to the lack of the first 817 bp of exon 2 that included the ATG start site. RT-PCR and western blot analysis of samples from various tissues detects no expression in tissues other than kidney and demonstrates approximately twice the level of both mRNA and protein in kidneys of 6-8 month old male transgenic vs. control mice. Immunohistochemical analysis using an anti-KAP monoclonal antibody showed the protein to be restricted to the proximal tubule cells of the kidney. However, at higher magnification "diffuse, intense staining" was apparent throughout transgenic male mice; control males exhibited less intense general staining.
(J:83177, J:95430, J:166496)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Nine transgenic founders were generated, from four of which (L-43, L-49, L-59 and L-64) transgenic lines were derived. Mice of lines L-49 and L-59 underwent detailed characterization. ( J:166496) |
| Original: |
J:166496 Tornavaca O, et al., Kidney androgen-regulated protein transgenic mice show hypertension and renal alterations mediated by oxidative stress. Circulation. 2009 Apr 14;119(14):1908-17 |
| All: |
6 reference(s) |
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Analysis Tools
