Gene Expression Data
Assay Details
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Assay
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Reference:
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J:34200
Henkemeyer M, et al., Nuk controls pathfinding of commissural axons in the mammalian central nervous system. Cell. 1996 Jul 12;86(1):35-46
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5792544
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Gene symbol:
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Ephb2
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Gene name:
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Eph receptor B2
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Specimens were fixed in 0.2% gluteraldehyde and post-fixed in formalin.
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Results
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Specimen
2F:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
3A +/lacZ:
embryonic day 10.0; Ephb2tm2Paw/Ephb2 +
(more  )
Specimen
3A +/lacZ:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2+
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3A lacZ/lacZ:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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4 somites
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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4 somites
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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4 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
3E
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Expression is in longitudinal stripes along the length of the brain.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3G E9.5:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3G E10:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3G E10.5:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Very strong |
Regionally restricted |
3H
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Expression is in the tegmental region.
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Specimen
3I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: diencephalon |
Very strong |
Regionally restricted |
3J
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Expression is in the preoptic and the hypothalamus areas.
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| TS17: midbrain |
Very strong |
Regionally restricted |
3J
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Expression is in the tegmental region.
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| TS17: hindbrain |
Present |
Regionally restricted |
3J
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Expression is ventral.
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Specimen
3K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: cranial nerve |
Present |
Regionally restricted |
3K
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Expression is in the oculomotor nerve.
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| TS17: diencephalon |
Present |
Regionally restricted |
3K
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Expression is in the preoptic and the hypothalamus areas.
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| TS17: midbrain |
Present |
Regionally restricted |
3K
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Expression is ventral.
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Specimen
3L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
7A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
7B:
embryonic day 14.5; Ephb2tm2Paw/ Ephb2tm2Paw
(more )
Note: (D) is a higher magnification of the boxed area in (B).
Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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(D) is a higher magnification of the boxed area in (B).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
7D
,
7B
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Expression is in cells underneath the anterior commissure pars posterior tract and above the growth cones that accumulate prior to midline fusion.
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Specimen
7C:
embryonic day 14.5; Ephb2tm2Paw/ Ephb2tm2Paw
(more )
Note: (F,G) are higher magnifications of the boxed area in (C).
Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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(F,G) are higher magnifications of the boxed area in (C).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
7G
,
7F
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7C
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Expression is ventral in the posterior hypothalamus in cells underneath the anterior commissure pars posterior tract and above the growth cones that accumulate prior to midline fusion.
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Specimen
7E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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