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Gene Expression Data
Assay Details
Assay
Reference: J:137253 Plachez C, et al., Nuclear factor I gene expression in the developing forebrain. J Comp Neurol. 2008 May 20;508(3):385-401
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5792126
Gene symbol: Nfia
Gene name: nuclear factor I/A
Antibody: Anti-NFIA
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1H: postnatal; Nfiatm1Rmg/Nfiatm1Rmg (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS28: brain Absent 1H
Specimen 1I: postnatal (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS28: brain Present 1I
Specimen 2I/I': embryonic day 18.5 (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS26: neocortex Present 2I' , 2I
TS26: cerebral cortex subventricular zone Present 2I' , 2I Expressed at lower levels compared to in the neocortex.
TS26: cerebral cortex ventricular zone Present 2I' , 2I Expressed at lower levels compared to in the neocortex.
Specimen 5E/E'/E": postnatal day 5 (more )
Note: Vibratome section. Double labeled: red - Nfia; green - Rbfox3 (assay MGI:5792535).
Structure Level Pattern Image Note
TS28: cortical layer IV Present 5E left , 5E merge Expression did not colocalize with Rbfox3.
TS28: cortical layer V Present 5E left , 5E merge Expression did not colocalize with Rbfox3.
TS28: cortical layer II Present 5E' left , 5E left , 5E' merge , 5E merge Expression did not colocalize with Rbfox3.
TS28: cortical layer III Present 5E' left , 5E left , 5E' merge , 5E merge Expression did not colocalize with Rbfox3.
TS28: cortical layer VI Present 5E" left , 5E left , 5E merge , 5E" merge Most positive cells colocalized with Rbfox3.
Specimen 5G/G'/G": embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Double labeled: red - Nfia; green - Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: cerebral cortex marginal zone Present 5G' , 5G Expression did not colocalize with Gad1 (GABAergic neurons).
TS26: cortical plate Present 5G" , 5G Expression did not colocalize with Gad1 (GABAergic neurons).
Specimen 9E/E': embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Double labeled: red - Nfia; green - Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: prethalamus Present Scattered 9E , 9E' A small number of the cells were double-labeled with GAD1.
TS26: diencephalon Present Regionally restricted 9E' Expressed in cells lining the third ventricle.
TS26: hypothalamus Present 9E
Specimen not shown: embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Also stained for Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: dentate gyrus Present Expression did not colocalize with Gad1 (GABAergic neurons).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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