About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:137253 Plachez C, et al., Nuclear factor I gene expression in the developing forebrain. J Comp Neurol. 2008 May 20;508(3):385-401
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5792126
Gene symbol: Nfia
Gene name: nuclear factor I/A
Antibody: Anti-NFIA
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1H: postnatal; Nfiatm1Rmg/Nfiatm1Rmg (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS28: brain Absent 1H
Specimen 1I: postnatal (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS28: brain Present 1I
Specimen 2I/I': embryonic day 18.5 (more )
Note: Vibratome section.
Structure Level Pattern Image Note
TS26: neocortex Present 2I' , 2I
TS26: cerebral cortex subventricular zone Present 2I' , 2I Expressed at lower levels compared to in the neocortex.
TS26: cerebral cortex ventricular zone Present 2I' , 2I Expressed at lower levels compared to in the neocortex.
Specimen 5E/E'/E": postnatal day 5 (more )
Note: Vibratome section. Double labeled: red - Nfia; green - Rbfox3 (assay MGI:5792535).
Structure Level Pattern Image Note
TS28: cortical layer IV Present 5E left , 5E merge Expression did not colocalize with Rbfox3.
TS28: cortical layer V Present 5E left , 5E merge Expression did not colocalize with Rbfox3.
TS28: cortical layer II Present 5E' left , 5E left , 5E' merge , 5E merge Expression did not colocalize with Rbfox3.
TS28: cortical layer III Present 5E' left , 5E left , 5E' merge , 5E merge Expression did not colocalize with Rbfox3.
TS28: cortical layer VI Present 5E" left , 5E left , 5E merge , 5E" merge Most positive cells colocalized with Rbfox3.
Specimen 5G/G'/G": embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Double labeled: red - Nfia; green - Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: cerebral cortex marginal zone Present 5G' , 5G Expression did not colocalize with Gad1 (GABAergic neurons).
TS26: cortical plate Present 5G" , 5G Expression did not colocalize with Gad1 (GABAergic neurons).
Specimen 9E/E': embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Double labeled: red - Nfia; green - Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: prethalamus Present Scattered 9E , 9E' A small number of the cells were double-labeled with GAD1.
TS26: diencephalon Present Regionally restricted 9E' Expressed in cells lining the third ventricle.
TS26: hypothalamus Present 9E
Specimen not shown: embryonic day 18.5; Gad1tm1.1Tama/Gad1+ (more )
Note: Vibratome section. Also stained for Gad1 reporter (assay MGI:5792145).
Structure Level Pattern Image Note
TS26: dentate gyrus Present Expression did not colocalize with Gad1 (GABAergic neurons).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory