Antibody:	Anti-NFIA
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: brain	Absent		1D

Structure	Level	Pattern	Image	Note
TS28: brain	Present		1E

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2A , 2A'	Expressed in the roofplate.

Structure	Level	Pattern	Image	Note
TS21: cortical preplate	Present		2C , 2C'

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Regionally restricted	2E' , 2E	Expressed in layers IV, V, and VI.
TS23: cerebral cortex marginal layer	Present		2E' , 2E
TS23: cerebral cortex subventricular zone	Present		2E' , 2E
TS23: cerebral cortex ventricular layer	Present		2E' , 2E
TS23: cortical subplate	Present		2E' , 2E

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex marginal zone	Present		2G' , 2G
TS25: cerebral cortex subventricular zone	Present		2G' , 2G
TS25: cerebral cortex ventricular zone	Present		2G' , 2G
TS25: cortical subplate	Present		2G' , 2G
TS25: neocortex	Present	Regionally restricted	2G , 2G'	Expressed in layer VI.

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Strong		3A , 3A'
TS27: cortical layer III	Strong		3A , 3A'
TS27: cortical layer V	Strong		3A , 3A'
TS27: cortical layer VI	Strong		3A , 3A'

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		3C' , 3C	Expression began to decline, particularly in the upper layers.
TS28: cortical layer III	Present		3C' , 3C	Expression began to decline, particularly in the upper layers.
TS28: cortical layer V	Present		3C' , 3C	Expression began to decline, particularly in the upper layers.
TS28: cortical layer VI	Present		3C' , 3C	Expression began to decline, particularly in the upper layers.

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Present		3E , 3E'	Reduced expression.
TS28: piriform cortex	Present		3E , 3E'

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Present		3G' , 3G

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Uniform	4A' , 4A

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Uniform	4C , 4C'

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present	Uniform	4E , 4E'

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Non-Uniform	4G" , 4G , 4G' , 4G'"	Lower level expression in rostral compared to caudal.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Non-Uniform	4I' , 4I'" , 4I , 4I"	Lower level expression in rostral compared to caudal.

Structure	Level	Pattern	Image	Note
TS23: neocortex	Strong	Regionally restricted	8A	Expressed in the cingulate cortex.
TS23: cerebral cortex	Strong	Regionally restricted	8A	Expressed in the cingulate cortex. More widely expressed throughout the ventricular zone and in cells of the indusium griseum and the glial wedge compared to NFIB.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	8C	Expressed in the indusium griseum, the glial wedge, and also in the subcallosal sling.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	8E	Expressed in the indusium griseum, the glial wedge, and also in the subcallosal sling.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex ventricular zone	Present		8G
TS28: cerebral cortex	Present	Regionally restricted	8G	Expression at the midline was higher than NFIB expression.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		8I
TS28: cerebral cortex ventricular zone	Present		8I
TS28: cerebral cortex	Present	Regionally restricted	8I' , 8I	Expressed in the indusium griseum. Present more dorsomedially than the NFIB-expressing cells.

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		9A
TS20: telencephalon	Present	Regionally restricted	9A	Expressed in amygdala.
TS20: optic chiasma	Weak		9A' , 9A
TS20: thalamus	Weak	Regionally restricted	9A	Expressed in posterior thalamus.
TS20: hypothalamus	Weak		9A

Structure	Level	Pattern	Image	Note
TS21: prethalamus	Present		9C
TS21: optic chiasma	Weak		9C' , 9C
TS21: telencephalon	Present	Regionally restricted	9C	Expressed in amygdala.
TS21: thalamus	Weak	Regionally restricted	9C	Expressed in posterior thalamus.
TS21: hypothalamus	Weak		9C

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	10A' , 10A	Expressed in the hippocampal primordium.

Structure	Level	Pattern	Image	Note
TS23: hippocampus	Present	Widespread	10C' , 10C	Expressed throughout the layers.

Structure	Level	Pattern	Image	Note
TS25: dentate gyrus	Present		10E , 10E'
TS25: hippocampus	Present	Regionally restricted	10E' , 10E	Expressed in both the stratum oriens and the stratum pyramidale. No expression in the stratum radiatum.

Structure	Level	Pattern	Image	Note
TS28: dorsal thalamus	Present		11A
TS28: CA3 pyramidal cell layer	Present		11A , 11A' , 11A"	More highly expressed in CA3 compared to CA2.
TS28: CA2 pyramidal cell layer	Present		11A" , 11A' , 11A	Expression in CA2 was higher than in CAl.
TS28: CA1 pyramidal cell layer	Present		11A , 11A'
TS28: dentate gyrus	Strong		11A' , 11A'" , 11A
TS28: hippocampus pyramidal cell layer	Present	Regionally restricted	11A'	Expressed in the stratum pyramidale.

Structure	Level	Pattern	Image	Note
TS28: dorsal thalamus	Absent		11C
TS28: dentate gyrus	Strong		11C
TS28: CA1 pyramidal cell layer	Present		11C , 11C'	Increased expression.
TS28: CA2 pyramidal cell layer	Present		11C' , 11C	Decreased expression.
TS28: CA3 pyramidal cell layer	Present		11C' , 11C	Decreased expression.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		11E , 11E'
TS28: CA1 pyramidal cell layer	Present		11E' , 11E	Increased expression.
TS28: CA2 pyramidal cell layer	Present		11E , 11E'	Decreased expression.
TS28: CA3 pyramidal cell layer	Present		11E , 11E'	Decreased expression.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Weak	Regionally restricted	11G , 11G' , 11G'"	Expressed in the inner granular layer.
TS28: CA2 pyramidal cell layer	Weak		11G" , 11G , 11G'
TS28: CA3 pyramidal cell layer	Weak		11G" , 11G , 11G'