Antibody:	aHox2.1a
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: neural plate	Absent		1A
TS13: surface ectoderm	Absent		1A
TS13: neural crest	Absent		1A	No expression in presumptive neural crest cell mass emerging from the neural plate.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Absent		1B	No expression in neural crest cells migrating to occipital level along dorsolateral pathway just beneath surface ectoderm.
TS14: surface ectoderm	Present	Regionally restricted	1B	Expression in the presumptive nodose placode.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord	Strong	Regionally restricted	1C
TS14: mesenchyme derived from lateral plate	Strong	Regionally restricted	1C
TS14: trunk somite	Strong	Regionally restricted	1C

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted	1D	Expression in lateral mesodermal cells surrounding the foregut.

Structure	Level	Pattern	Image	Note
TS14: surface ectoderm	Present	Regionally restricted	1E	Expression in the future nodose placode.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	2B , 2A	Expression in presumptive neural crest cells scattered between surface ectoderm and cephalic vein corresponding to the dorsolateral migratory pathway. Distribution of expressing migratory cells extends more dorsoanterior than expressing cells of the surface ectoderm.
TS15: branchial arch	Present	Regionally restricted	2A , 2B	Expression in cells localized within and posterior to pharyngeal arch 4.
TS15: surface ectoderm	Present	Regionally restricted	2B , 2A	Expression within the area of pharygeal arch 6 continues caudually to a ridge in the surface ectoderm encircling the posterior portion of the pharynx (circumpharyngeal ridge.)
TS15: trunk mesenchyme	Present	Regionally restricted	2B , 2A	Expression in presumptive crest cells localizing beyond the caudal limit of the pharyngeal apparatus, making an arch along the cardinal vein within the lateral body wall.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present	Regionally restricted	2D	Expression in pharyngeal pouch 4.
TS15: neural crest	Present	Regionally restricted	2D	Expression in several cells between the nodose placode and cephalic vein. Expression in in cells caudal to the area of pharyngeal arch 6, extending medially to the lateral aspect of the foregut.

Structure	Level	Pattern	Image	Note
TS15: neural crest	Present	Regionally restricted	2E	Expression in cells caudal to the area of pharyngeal arch 6, extending medially to lateral aspect of the foregut. Expression in cells between the nodose placode and cephalic vein.
TS15: surface ectoderm	Present	Regionally restricted	2E	Expression in nodose placode.

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present	Regionally restricted	3A	Expression in pharyngeal ectoderm in the area of the future nodose placode.
TS15: migrating neural crest	Present	Regionally restricted	3A	Expression in presumptive vagal crest cells migrating through the dorsolateral pathway lateral to the cardinal vein.

Structure	Level	Pattern	Image	Note
TS15: neural crest	Present	Regionally restricted	3B	Expression in mesenchymal cells (presumptive neural crest cells) more dorsal than those of the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS16: vagus ganglion	Present	Regionally restricted	4A	Expression in nodose ganglion (inferior vagus ganglion) at the anterior-most level.
TS16: surface ectoderm	Absent		4A	No expression in ectodermal cells.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	4B	Expression in neuroblasts in the external limiting membrane.

Structure	Level	Pattern	Image	Note
TS16: 3rd branchial groove ectoderm	Present	Regionally restricted	4C	Expression in nodose placode.
TS16: vagus ganglion	Present	Regionally restricted	4C	Expression in nodose ganglion (inferior vagus ganglion).

Structure	Level	Pattern	Image	Note
TS16: 3rd branchial groove ectoderm	Present	Regionally restricted	4D	Expression in nodose placode.
TS16: migrating neural crest	Present	Regionally restricted	4D	Expression in a few mesenchymal cells between the nodose placone and nodose ganglion - either migrating vagal crest cells or cells migrating from placode giving rise to nodose neurons.

Structure	Level	Pattern	Image	Note
TS16: neural crest	Present	Regionally restricted	4E	Expression in independent population of mesenchymal cells extending surrounding foregut wall and extending laterally to the surface ectoderm thought to be derived from dorsolateral population of vagal crest contributing to nodose ganglion.
TS16: neural tube	Present	Regionally restricted	4E	Stronger expression in cells near the outer edges of the neural tube.

Structure	Level	Pattern	Image	Note
TS16: neural crest	Present	Regionally restricted	4F	Caudal to the level of the nodose ganglion, expression domain interrupted by the cardinal vein.
TS16: neural tube	Present	Regionally restricted	4F	Stronger expression in cells near the outer edges of the neural tube.

Structure	Level	Pattern	Image	Note
TS16: neural crest	Present	Regionally restricted	4G	Expression in cells within the body wall, form lower portion of wall corresponding to circumpharyngeal ridge.

Structure	Level	Pattern	Image	Note
TS17: mesenchyme derived from neural crest	Present	Regionally restricted	7A	Expression in postpharyngeal area. Caudal expression in cells making an arch which opens anteriorly and is continuous with the nodose ganglion.