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Tg(Gad2-tdTomato)DJhi
Transgene Detail
Summary
Symbol: Tg(Gad2-tdTomato)DJhi
Name: transgene insertion D, Johannes Hirrlinger
MGI ID: MGI:5790577
Synonyms: TgN(GAD65-tdTomato)
Transgene: Tg(Gad2-tdTomato)DJhi  Location: unknown  
Alliance: Tg(Gad2-tdTomato)DJhi page
Transgene
origin
Strain of Origin:  C57BL/6J
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsA sequence was engineered comprising the tdTomato red fluorescent protein open reading frame followed by the SV40 polyadenylation sequence and an FRT site-flanked neomycin resistance cassette. This was inserted into the BAC RP23-407K8 immediately downstream of the translation-initiating ATG in exon 1 of the mouse glutamic acid decarboxylase 2 gene (Gad2; also called Gad65); the BAC contains all 16 exons, 72 kb of 5'- and 64 kb of 3'-flanking DNA. The neo cassette was removed by flp-mediated recombination. Of 11 founder mouse lines that had integrated the transgene and that transmitted it through the germline, only one exhibited the expected expression pattern upon fluorescence imaging of brain sections. (J:233808)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:233808 Besser S, et al., A Transgenic Mouse Line Expressing the Red Fluorescent Protein tdTomato in GABAergic Neurons. PLoS One. 2015;10(6):e0129934
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory