Probe:	inka2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		3A
TS17: dorsal root ganglion	Present		3A
TS17: facial ganglion	Present		3A
TS17: migrating neural crest	Present		3A
TS17: telencephalon	Present		3A
TS17: trigeminal ganglion	Present		3A

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		3C

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		3D

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		3E

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		3F
TS17: facial ganglion	Present		3F
TS17: trigeminal ganglion	Present		3F
TS17: telencephalon	Present	Scattered	3F	Expression is in the superficial thin layer of the telencephalon, absent in neuroepithelial cells.
TS17: telencephalon ventricular layer	Absent		3F

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Scattered	3G	Expression is in the superficial thin layer of the telencephalon, absent in neuroepithelial cells.
TS17: telencephalon ventricular layer	Absent		3G

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Strong	Regionally restricted	3H	Expression is in narrow stripes adjacent to the floor plate.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Strong	Regionally restricted	3I	Expression is in narrow stripes adjacent to the floor plate.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4A	Expression is in the surface area covering the entire telencephalic vesicle and in the bed nucleus of stria terminalis.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4B	Expression is in the surface area covering the entire telencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Strong	Regionally restricted	4C	Expression is in the superficial layer of the medial telencephalic wall and the caudal telencephalic wall covering the hippocampus region.
TS22: telencephalon septum	Present	Regionally restricted	4C	Expression is in the bed nucleus of stria terminalis.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		4D	Expression is in the superficial layer of the medial telencephalic wall and the caudal telencephalic wall covering the hippocampus region.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata ventricular layer	Present	Regionally restricted	4E	Expression is ventral.
TS22: hindbrain	Present	Regionally restricted	4E	Expression is in scattered cells over the ventral and lateral parenchyma of the hindbrain.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata ventricular layer	Present	Regionally restricted	4G , 4F	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS24: medulla oblongata	Present		4H
TS24: pons	Present		4H
TS24: telencephalon	Present	Regionally restricted	4H	Expression is in the anterior part and is absent in the superficial layer.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Single cells	4I	There are sparsely distributed positive cells across the cortical layers, absent on the cortical surface.

Structure	Level	Pattern	Image	Note
TS24: forebrain	Present	Regionally restricted	4J	Expression is in the ventral forebrain, absent on the cortical surface.
TS24: thalamus	Present		4K , 4J	Expression is in the ventral thalamus.
TS24: amygdala	Present		4J
TS24: basal ganglia	Present		4J
TS24: hypothalamus	Present		4J
TS24: piriform cortex	Present		4J
TS24: telencephalon septum	Present		4J

Structure	Level	Pattern	Image	Note
TS21: hindbrain ventricular layer	Present	Regionally restricted	5C , 5A	Expression is in the ventral ventricular zone surrounding the 4th ventricle.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	5G , 5E	Expression is ventral in the thoracic spinal cord.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Diffuse	6A
TS28: hippocampus	Strong		6A
TS28: cortical layer II	Present		6B , 6C , 6A
TS28: cortical layer III	Present		6B , 6C , 6A
TS28: cortical layer I	Present	Single cells	6C , 6B , 6A

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Strong		6D

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present		7A
TS28: bed nucleus of stria terminalis	Present		7A
TS28: caudate-putamen	Present		7A
TS28: cingulate cortex	Present		7A
TS28: entorhinal cortex	Present		7A
TS28: neocortex	Present		7A
TS28: piriform cortex	Present		7A
TS28: telencephalon septum	Present		7A

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		7B
TS28: cortical layer III	Present		7B
TS28: cortical layer IV	Present		7B
TS28: cortical layer V	Present		7B
TS28: cortical layer VI	Present		7B
TS28: cerebral hemisphere white matter	Absent		7B
TS28: corpus callosum	Absent		7B
TS28: cortical layer I	Absent		7B

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		7C
TS28: cortical layer I	Absent		7C

Structure	Level	Pattern	Image	Note
TS28: amygdaloid nucleus	Present		7D
TS28: hippocampus	Present		7D
TS28: epithalamus	Absent		7D
TS28: globus pallidus	Absent		7D
TS28: hypothalamus	Absent		7D
TS28: thalamus	Absent		7D

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Present		7E
TS28: globus pallidus	Absent		7E

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present		7F
TS28: CA2 pyramidal cell layer	Present		7F
TS28: CA3 pyramidal cell layer	Present		7F
TS28: dentate gyrus granule cell layer	Present		7F

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present		7G
TS28: CA2 pyramidal cell layer	Present		7G
TS28: CA3 pyramidal cell layer	Present		7G

Structure	Level	Pattern	Image	Note
TS28: anterior commissure	Absent		7H
TS28: corpus callosum	Absent		7H
TS28: posterior commissure	Absent		7H

Structure	Level	Pattern	Image	Note
TS28: midbrain	Absent		7I

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Trace		7J	Absent in the rest of the cerebellum.
TS28: medulla oblongata	Absent		7J

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		8A
TS20: spinal cord	Absent		8A

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	8E , 8B	Expression is in a subset of neuroepithelial cells in the ventral and caudal parts of the spinal cord adjacent to the floor plate.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	8C	Expression is in a subset of neuroepithelial cells in the ventral and caudal parts of the spinal cord adjacent to the floor plate.

Structure	Level	Pattern	Image	Note
TS24: spinal cord gray matter	Present	Scattered	8D
TS24: spinal cord white matter	Present	Scattered	8D
TS24: dorsal root ganglion	Trace		8D

Structure	Level	Pattern	Image	Note
TS24: spinal cord gray matter	Present	Scattered	8F
TS24: spinal cord white matter	Present	Scattered	8F

Structure	Level	Pattern	Image	Note
TS22: cerebellum layer	Present	Regionally restricted	9A	Expression is in the external granule layer.

Structure	Level	Pattern	Image	Note
TS22: cerebellum layer	Present	Regionally restricted	9B	Expression is in the external granule layer.

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Present		9C

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Present		9D

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		9E

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		9F
TS28: cerebellum molecular layer	Present	Regionally restricted	9F	Expression is in small cells residing in the vicinity of Purkinje cells at the outermost region of the molecular layer.
TS28: cerebellum Purkinje cell layer	Absent		9F

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	10A	Expression is in the basal compartment.

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted	10C , 10B	Expression is in the superficial layer of the olfactory bulb with little or no expression in the olfactory subventricular zone.
TS24: rostral migratory stream	Ambiguous		10B , 10C	Little or no expression in rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present	Regionally restricted	10D , 10E	Expression is in periglomerular cells.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Absent

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Present
TS28: entorhinal cortex	Present